Human CXCL6/GCP-2 Antibody

Catalog #: AF333
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF333
AF333-SP

Chemotaxis Induced by CXCL6/GCP‑2 and Neutralization by Human CXCL6/GCP‑2 Antibody.

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Human CXCL6/GCP-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL6/GCP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) MIP-2, rmKC, recombinant rat (rr) CINC-2 alpha, rmCRG-2, recombinant human (rh) NAP-2, rrCINC-2 beta and rhMCP-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CXCL6/GCP-2
Val40-Asn114
Accession # P80162

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CXCL6/GCP‑2 (Catalog # 333-GC)

Neutralization

Measured by its ability to neutralize CXCL6/GCP‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND₅₀) is typically 0.8-4.0 µg/mL in the presence of 80 ng/mL Recombinant Human CXCL6/GCP‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL6/GCP‑2 and Neutralization by Human CXCL6/GCP‑2 Antibody. Recombinant Human CXCL6/ GCP-2 (Catalog # 333-GC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL6/ GCP-2 (80 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CXCL6/GCP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF333). The ND₅₀ is typically 0.8-4.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL6/GCP-2

GCP-2 (granulocyte chemotactic protein-2) also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level.

Human GCP-2 cDNA encodes a propeptide of 114 amino acid residues with a predicted 37 aa residue signal peptide and 77 aa residue mature protein. Several forms of natural GCP-2 have been isolated from MG-63 conditioned media, indicating that GCP-2 undergoes limited processing at both the N- and C-termini. Human GCP-2 is a primary response gene whose induction by cytokines is attenuated by dexamethasone.

Human GCP-2 and mouse GCP-2/LIX have been shown to chemoattract and activate neutrophils, but not eosinophils and monocytes. It is likely that GCP-2 activities are mediated via the human or mouse CXCR2.

References

Proost, P. et al. (1993) J. Immunol. 150:1000.
Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
Wuyts, A. et al. (1997) J. Immunol. 157:1736.

Entrez Gene IDs

6372 (Human)

Alternate Names

chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); Chemokine alpha 3; CKA-3Granulocyte chemotactic protein 2; CXCL6; GCP-2; GCP2member 6 (granulocytechemotactic protein 2); GCP-2Small-inducible cytokine B6; Small inducible cytokine subfamily B (Cys-X-Cys), member b

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Citation for Human CXCL6/GCP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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