Human CXCR7/RDC-1 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
CXCR7/RDC‑1 in Human Squamous Cell Carcinoma. CXCR7/RDC-1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC-1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: CXCR7/RDC-1
The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.
Product Datasheets
Citations for Human CXCR7/RDC-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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CXCR4-CXCL12-CXCR7 and PD-1/PD-L1 in Pancreatic Cancer: CXCL12 Predicts Survival of Radically Resected Patients
Authors: C D'Alterio, A Giardino, G Scognamigl, G Butturini, L Portella, G Guardascio, I Frigerio, M Montella, S Gobbo, G Martignoni, V Napolitano, F De Vita, F Tatangelo, R Franco, S Scala
Cells, 2022-10-22;11(21):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Biological Role of Tumor/Stromal CXCR4-CXCL12-CXCR7 in MITO16A/MaNGO-OV2 Advanced Ovarian Cancer Patients
Authors: C D'Alterio, A Spina, L Arenare, P Chiodini, M Napolitano, F Galdiero, L Portella, V Simeon, S Signoriell, F Raspaglies, D Lorusso, C Pisano, N Colombo, GF Zannoni, NS Losito, R De Cecio, G Scognamigl, D Califano, D Russo, V Tuninetti, MC Piccirillo, P Gargiulo, F Perrone, S Pignata, S Scala
Cancers, 2022-04-06;14(7):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis
Authors: SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn
International Journal of Molecular Sciences, 2022-01-14;23(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides
Authors: M Meyrath, M Szpakowska, J Zeiner, L Massotte, MP Merz, T Benkel, K Simon, J Ohnmacht, JD Turner, R Krüger, V Seutin, M Ollert, E Kostenis, A Chevigné
Nat Commun, 2020-06-19;11(1):3033.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking
Authors: N Pluchino, R Mamillapal, S Shaikh, S Habata, A Tal, M Gaye, HS Taylor
J. Cell. Mol. Med., 2020-01-06;24(4):2464-2474.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells
Authors: C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala
Cell Death Dis, 2019-07-22;10(8):562.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer
Authors: S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu
Cancer Res., 2019-04-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
CXCR7 promotes melanoma tumorigenesis via Src kinase signaling
Authors: S Xu, J Tang, C Wang, J Liu, Y Fu, Y Luo
Cell Death Dis, 2019-02-25;10(3):191.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer
Authors: M Wang, X Yang, M Wei, Z Wang
J Cancer, 2018-10-10;9(21):3898-3903.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration
Authors: E Willems, M Dedobbelee, M Digregorio, A Lombard, N Goffart, PN Lumapat, J Lambert, P Van den Ac, M Szpakowska, A Chevigné, F Scholtes, B Rogister
Oncogene, 2018-08-06;0(0):.
Species: Human
Sample Types: Transfected Whole Cells
Applications: Cell Selection -
G-Protein-Coupled Receptor CXCR7 Is Overexpressed in Human and Murine Endometriosis
Authors: N Pluchino, R Mamillapal, I Moridi, R Tal, HS Taylor
Reprod Sci, 2018-03-27;0(0):1933719118766.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells
Authors: T D'huys, S Claes, T Van Loy, D Schols
Heliyon, 2018-03-01;4(3):e00557.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, Inhibition -
Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging
Authors: N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker
J. Biol. Chem., 2017-11-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function
Authors: YH Cheng, JM Eby, HM LaPorte, BF Volkman, M Majetschak
PLoS ONE, 2017-11-10;12(11):e0187949.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
Authors: M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang
Sci Rep, 2017-08-15;7(1):8289.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells
Authors: Vashendriya V V Hira
Biochim. Biophys. Acta, 2016-12-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing.
Authors: Muhlethaler-Mottet A, Liberman J, Ascencao K, Flahaut M, Balmas Bourloud K, Yan P, Jauquier N, Gross N, Joseph J
PLoS ONE, 2015-05-08;10(5):e0125616.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4.
Authors: Walsh T, Harper M, Poole A
Cell Signal, 2014-10-05;27(1):37-46.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells.
Authors: Liu Y, Carson-Walter E, Walter K
PLoS ONE, 2014-08-01;9(8):e103938.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Neutralization -
COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
Authors: Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F
BMC Cancer, 2014-06-06;14(0):407.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats.
Authors: Walters, M J, Ebsworth, K, Berahovich, R D, Penfold, M E T, Liu, S-C, Al Omran, R, Kioi, M, Chernikova, S B, Tseng, D, Mulkearns-Hubert, E E, Sinyuk, M, Ransohoff, R M, Lathia, J D, Karamchandani, J, Kohrt, H E K, Zhang, P, Powers, J P, Jaen, J C, Schall, T J, Merchant, M, Recht, L, Brown, J M
Br J Cancer, 2014-01-14;110(5):1179-88.
Species: Human, Rat
Sample Types: Whole Tissue
Applications: IHC, IHC-P -
Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis.
Authors: Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S
Nature, 2013-11-20;505(7481):97-102.
Species: Human, Mouse
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC, Immunoprecipitation, Western Blot -
Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
Authors: Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N
PLoS ONE, 2012-08-20;7(8):e43665.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Ubiquitination of CXCR7 controls receptor trafficking.
Authors: Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R
PLoS ONE, 2012-03-23;7(3):e34192.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay, ICC -
CXCR7 functions as a scavenger for CXCL12 and CXCL11.
Authors: Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M
PLoS ONE, 2010-02-11;5(2):e9175.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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