Human Cyclin D1 Antibody

Name: Human Cyclin D1 Antibody
Brand: R&D Systems
SKU: MAB4314
Rating: 5 (1 reviews)

Catalog #: MAB4314
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4314
MAB4314-SP

1 Image

All Cyclin D1 Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cyclin D1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cyclin D1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 328303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Cyclin D1
Met1-Ile295
Accession # P24385

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human heart

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

Cyclin D1 in COLO 205 Human Cell Line. Cyclin D1 was detected in immersion fixed COLO 205 human colorectal adenocarcinoma cell line using Mouse Anti-Human Cyclin D1 Monoclonal Antibody (Catalog # MAB4314) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin D1

Cyclin D1 is a 44 kDa cell cycle regulatory protein whose expression level and nuclear/cytoplasmic distribution are tightly regulated in synchrony with the cell cycle. Cyclin D1 complexes containing Cdk4 or Cdk6 induce phosphorylation of Rb, a requirement for progression through the G1/S cell cycle transition. Human Cyclin D1 shares 93% amino acid sequence identity with mouse and rat Cyclin D1.

Entrez Gene IDs

595 (Human); 12443 (Mouse); 58919 (Rat)

Alternate Names

B-cell lymphoma 1 protein; BCL-1 oncogene; Bcl-1; BCL1D11S287E; CCND1; cyclin D1 (PRAD1: parathyroid adenomatosis 1); Cyclin D1; Cyl-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1 oncogene; PRAD1; PRAD1B-cell CLL/lymphoma 1; U21B31

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Cyclin D1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1