Human Cystatin C Antibody

Name: Human Cystatin C Antibody
Brand: R&D Systems
SKU: MAB11962
Rating: 5 (2 reviews)

Catalog #: MAB11962
1 Citation 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11962-SP
MAB11962-500
MAB11962-100

All Cystatin C Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (2)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cystatin C Antibody Summary

Species Reactivity

Human

Specificity

Detects human Cystatin C in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human Cystatins A, B, D, E/M, F, S, SA, SN, X/Z/P, or recombinant mouse Cystatin C is observed.

Source

Monoclonal Mouse IgG_2B Clone # 197820

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cystatin C Ser27-Ala146 Accession # P01034

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Human Cystatin C Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human Cystatin C Biotinylated Antibody (Catalog # BAM11961)

Standard: Recombinant Human Cystatin C Protein, CF (Catalog # 1196-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 of the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant (Leu68 to Gln) is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

References

Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Alternate Names

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Cystatin C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort.
Authors: Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D
PLoS ONE, 2016-01-28;11(1):e0148073.
Species: Human
Sample Types: Plasma
Applications: ELISA Development