Human Cytokeratin 14 Antibody Summary
DGKVVSTHEQVLRTKN
Applications
Purkis, P. et al. (1990) J. Cell Sci. 97:39. This application was not tested by R&D Systems.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cytokeratin 14 in A431 Human Cell Line. Cytokeratin 14 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Cytokeratin 14 in NHEK Human Normal Epidermal Keratinocytes. Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Cytokeratin 14 in human skin. Cytokeratin 14 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 14
Cytokeratin 14, a type I intermediate filament protein, is one of the two keratins which distinguish stratifying epithelium from simple epithelium.
Product Datasheets
Citations for Human Cytokeratin 14 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Bronchioalveolar organoids: A preclinical tool to screen toxicity associated with antibody-drug conjugates
Authors: McCray, TN;Nguyen, V;Heins, JS;Nguyen, E;Stewart, K;Ford, CT;Neace, C;Gupta, P;Ortiz, DJ;
Toxicology and applied pharmacology
Species: Human hepegivirus
Sample Types: Organoid
Applications: Immunohistochemistry -
Decreased IGF type 1 receptor signaling in mammary epithelium during pregnancy leads to reduced proliferation, alveolar differentiation, and expression of insulin receptor substrate (IRS)-1 and IRS-2.
Authors: Sun Z, Shushanov S, LeRoith D, Wood TL
Endocrinology, 2011-05-31;152(8):3233-45.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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Anti-keratin 14 antibody was tested at 1:50 and 1:100 dilutions and the protocol includes citrate antigen retrieval. Pepsin and pronase were also used as alternative methods for antigen retrieval, without success. The antibody is not specific of the basal layer of the skin and targets all the layers.
Detection of keratin 14 by Simple Western (JESS) in reconstructed human epidermis lysate (about 200 µg/mL protein concentration).
Antibody dilution: 1/50
