Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody Summary
Asn2-Ile294
Accession # P49888
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 1E1/SULT1E1
Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
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Gong, H. et al. (2008) Cancer Res. 68:7386.
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Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
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Miksits, M. et al. 2005, Xenobiotica 35:1101.
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Takase, Y. et al. (2007) The Prostate 67:405.
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Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
- Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
- MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
- Wu, Z.L., et al. (2002) Faseb J. 16:539.
Product Datasheets
Citation for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Phiclust: a clusterability measure for single-cell transcriptomics reveals phenotypic subpopulations
Authors: M Mircea, M Hochane, X Fan, SM Chuva de S, D Garlaschel, S Semrau
Genome Biology, 2022-01-10;23(1):18.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
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Primary ab dilution 1:2000 (Maybe working with 1:4000)
40 ug protein per lane
