Human DC-SIGN/CD209 Antibody
Human DC-SIGN/CD209 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of DC‑SIGN in Human DC‑SIGN Transfected 3T3 Mouse Cell Line by Flow Cytometry. Human DC-SIGN and DC-SIGN2 transfected 3T3 mouse embryonic fibroblast cell line were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161, filled histograms) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Detection of DC‑SIGN in Human Monocyte Derived Dendritic Cells by Flow Cytometry. Human monocyte derived dendritic cells were stained with Mouse Anti-Human DC-SIGN Monoclonal Antibody (Catalog # MAB161) followed by PE-conjugated anti-mouse IgG (Catalog # F0102B) and Anti-Human B7-2/CD86 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB141F). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DC-SIGN/CD209
Human DC-Sign (dendritic cell-specific ICAM-3 grabbing nonintegrin; also CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).
- Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
- Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
- Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
- Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
- Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
- Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
- Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
- Baribaud, F. et al. (2001) J. Virol. 75:10281.
- Wu, L. et al. (2002) J. Virol. 76:5905.
- Baribaud, F. et al. (2002) J. Virol.76:9135.
Product Datasheets
Citations for Human DC-SIGN/CD209 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Suppression of DC-SIGN and gH Reveals Complex, Subset-Specific Mechanisms for KSHV Entry in Primary B Lymphocytes
Authors: N Palmerin, F Aalam, R Nabiee, M Muniraju, JG Ogembo, J Totonchy
Viruses, 2021-07-31;13(8):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression of the human or porcine C-type lectins DC-SIGN/L-SIGN confers susceptibility to porcine epidemic diarrhea virus entry and infection in otherwise refractory cell lines
Authors: P Zhao, LD Xu, Y Zhang, H Cao, R Chen, B Wang, YW Huang
Microbial pathogenesis, 2021-05-19;157(0):104956.
Species: Hamster
Sample Types: Whole Cells
Applications: IHC -
Early Colorectal Responses to HIV-1 and Modulation by Antiretroviral Drugs
Authors: C Herrera, MD McRaven, KG Laing, J Dennis, TJ Hope, RJ Shattock
Vaccines, 2021-03-08;9(3):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Blockade of DC-SIGN+ tumor-associated macrophages reactivates anti-tumor immunity and improves immunotherapy in muscle-invasive bladder cancer
Authors: B Hu, Z Wang, H Zeng, Y Qi, Y Chen, T Wang, J Wang, Y Chang, Q Bai, Y Xia, Y Wang, L Liu, Y Zhu, B Dai, J Guo, L Xu, W Zhang, J Xu
Cancer Res., 2020-02-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Dengue viruses infect human megakaryocytes, with probable clinical consequences
Authors: MB Vogt, A Lahon, RP Arya, JL Spencer Cl, R Rico-Hesse
PLoS Negl Trop Dis, 2019-11-25;13(11):e0007837.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cell-to-Cell Spreading of HIV-1 in Myeloid Target Cells Escapes SAMHD1 Restriction
Authors: M Xie, H Leroy, R Mascarau, M Woottum, M Dupont, C Ciccone, A Schmitt, B Raynaud-Me, C Vérollet, J Bouchet, L Bracq, S Benichou
MBio, 2019-11-19;10(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tricarboxylic Acid Cycle Activity and Remodeling of Glycerophosphocholine Lipids Support Cytokine Induction in Response to Fungal Patterns
Authors: S Márquez, JJ Fernández, C Mancebo, C Herrero-Sá, S Alonso, TA Sandoval, M Rodríguez, JR Cubillos-R, O Montero, N Fernández, M Sánchez Cr
Cell Rep, 2019-04-09;27(2):525-536.e4.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-?
Authors: KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten
Eur. J. Immunol, 2017-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Kluyveromyces marxianus and Saccharomyces boulardii Induce Distinct Levels of Dendritic Cell Cytokine Secretion and Significantly Different T Cell Responses In Vitro
PLoS ONE, 2016-11-29;11(11):e0167410.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Metformin Uniquely Prevents Thrombosis by Inhibiting Platelet Activation and mtDNA Release
Sci Rep, 2016-11-02;6(0):36222.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Clearance of autophagy-associated dying retinal pigment epithelial cells - a possible source for inflammation in age-related macular degeneration
Cell Death Dis, 2016-09-08;7(9):e2367.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
Sci Rep, 2016-07-07;6(0):29447.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Paracoccidioides brasiliensis interferes on dendritic cells maturation by inhibiting PGE2 production.
Authors: Fernandes R, Bachiega T, Rodrigues D, Golim M, Dias-Melicio L, Balderramas H, Kaneno R, Soares A
PLoS ONE, 2015-03-20;10(3):e0120948.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Porphyromonas gingivalis evasion of autophagy and intracellular killing by human myeloid dendritic cells involves DC-SIGN-TLR2 crosstalk.
Authors: El-Awady, Ahmed R, Miles, Brodie, Scisci, Elizabet, Kurago, Zoya B, Palani, Chithra, Arce, Roger M, Waller, Jennifer, Genco, Caroline, Slocum, Connie, Manning, Matthew, Schoenlein, Patricia, Cutler, Christop
PLoS Pathog, 2015-02-13;10(2):e1004647.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
PLoS Pathog, 2014-12-04;10(12):e1004548.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Live-attenuated measles virus vaccine targets dendritic cells and macrophages in muscle of nonhuman primates.
Authors: Rennick L, de Vries R, Carsillo T, Lemon K, van Amerongen G, Ludlow M, Nguyen D, Yuksel S, Verburgh R, Haddock P, McQuaid S, Duprex W, de Swart R
J Virol, 2014-12-03;89(4):2192-200.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Tissue
Applications: IHC -
Early biodistribution and persistence of a protective live attenuated SIV vaccine elicits localised innate responses in multiple lymphoid tissues.
Authors: Ferguson D, Mattiuzzo G, Ham C, Stebbings R, Li B, Rose N, Mee E, Smith D, Page M, Cranage M, Almond N, Towers G, Berry N
PLoS ONE, 2014-08-27;9(8):e104390.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Tissue
Applications: IHC-P -
Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients.
Authors: Beck, Zoltan, Jagodzinski, Linda L, Eller, Michael, Thelian, Doris, Matyas, Gary R, Kunz, Anjali N, Alving, Carl R
PLoS ONE, 2013-11-25;8(11):e81002.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
In vivo targeting of human DC-SIGN drastically enhances CD8(+) T-cell-mediated protective immunity.
Authors: Hesse C, Ginter W, Forg T, Mayer C, Baru A, Arnold-Schrauf C, Unger W, Kalay H, van Kooyk Y, Berod L, Sparwasser T
Eur J Immunol, 2013-07-22;43(10):2543-53.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
CLEC5A is critical for dengue virus-induced inflammasome activation in human macrophages.
Authors: Wu M, Chen S, Yang A, Lin W, Lin Y, Chen N, Tsai I, Li L, Hsieh S
Blood, 2012-11-14;121(1):95-106.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
Authors: Emara M, Royer PJ, Mahdavi J
J. Biol. Chem., 2011-12-28;287(8):5756-63.
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development -
Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
Authors: Johnson TR, McLellan JS, Graham BS
J. Virol., 2011-11-16;86(3):1339-47.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Semen clusterin is a novel DC-SIGN ligand.
Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, RodrÃgues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
J. Immunol., 2011-10-17;187(10):5299-309.
Species: Human
Sample Types: Seminal Plasma, Whole Cells
Applications: Flow Cytometry, Neutralization -
In situ distribution of HIV-binding CCR5 and C-type lectin receptors in the human endocervical mucosa.
Authors: Hirbod T, Kaldensjo T, Broliden K
PLoS ONE, 2011-09-30;6(9):e25551.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Plasmacytoid dendritic cells infiltrate the skin in positive tuberculin skin test indurations.
Authors: Bond E, Liang F, Sandgren KJ, Smed-Sorensen A, Bergman P, Brighenti S, Adams WC, Betemariam SA, Rangaka MX, Lange C, Wilkinson RJ, Andersson J, Lore K
J. Invest. Dermatol., 2011-08-18;132(1):114-23.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
DC-SIGN mediates cell-free infection and transmission of human T-cell lymphotropic virus type 1 by dendritic cells.
Authors: Jain P, Manuel SL, Khan ZK, Ahuja J, Quann K, Wigdahl B
J. Virol., 2009-08-19;83(21):10908-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC, Neutralization -
Gram-positive bacteria enhance HIV-1 susceptibility in Langerhans cells, but not in dendritic cells, via Toll-like receptor activation.
Authors: Ogawa Y, Kawamura T, Kimura T, Ito M, Blauvelt A, Shimada S
Blood, 2009-03-11;113(21):5157-66.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A role of the Ca2+ binding site of DC-SIGN in the phagocytosis of E. coli.
Authors: Iyori M, Ohtani M, Hasebe A, Totsuka Y, Shibata K
Biochem. Biophys. Res. Commun., 2008-10-10;377(2):367-72.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, ICC, Western Blot -
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
Mucosal Immunol, 2008-07-02;1(5):412-20.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Glycosylation-dependent interactions of C-type lectin DC-SIGN with colorectal tumor-associated Lewis glycans impair the function and differentiation of monocyte-derived dendritic cells.
Authors: Nonaka M, Ma BY, Murai R, Nakamura N, Baba M, Kawasaki N, Hodohara K, Asano S, Kawasaki T
J. Immunol., 2008-03-01;180(5):3347-56.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Lewis X oligosaccharides targeting to DC-SIGN enhanced antigen-specific immune response.
Authors: Wang J, Zhang Y, Wei J, Zhang X, Zhang B, Zhu Z, Zou W, Wang Y, Mou Z, Ni B, Wu Y
Immunology, 2007-03-20;121(2):174-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression of DC-SIGN and DC-SIGNR on human sinusoidal endothelium: a role for capturing hepatitis C virus particles.
Authors: Lai WK, Sun PJ, Zhang J, Jennings A, Lalor PF, Hubscher S, McKeating JA, Adams DH
Am. J. Pathol., 2006-07-01;169(1):200-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Recombinant vesicular stomatitis viruses encoding simian immunodeficiency virus receptors target infected cells and control infection.
Authors: Okuma K, Boritz E, Walker J, Sarkar A, Alexander L, Rose JK
Virology, 2005-12-01;346(1):86-97.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Lysates
Applications: Western Blot -
BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
Cancer Res., 2005-07-15;65(14):6409-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Urine dendritic cells: a noninvasive probe for immune activity in bladder cancer?
Authors: Beatty JD, Islam S, North ME, Knight SC, Ogden CW
BJU Int., 2004-12-01;94(9):1377-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
DC-SIGN-mediated infectious synapse formation enhances X4 HIV-1 transmission from dendritic cells to T cells.
Authors: Arrighi JF, Pion M, Garcia E, Escola JM, van Kooyk Y, Geijtenbeek TB, Piguet V
J. Exp. Med., 2004-11-15;200(10):1279-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
Blood, 2004-07-08;104(10):3267-75.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dendritic cells in the human decidua.
Authors: Gardner L, Moffett A
Biol. Reprod., 2003-06-25;69(4):1438-46.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr -
DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells.
Authors: Tailleux L, Schwartz O, Herrmann JL, Pivert E, Jackson M, Amara A, Legres L, Dreher D, Nicod LP, Gluckman JC, Lagrange PH, Gicquel B, Neyrolles O
J. Exp. Med., 2003-01-06;197(1):121-7.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Rhesus macaque dendritic cells efficiently transmit primate lentiviruses independently of DC-SIGN.
Authors: Wu L, 107253, Bashirova AA, Martin TD, Villamide L, Mehlhop E, Chertov AO, Unutmaz D, Pope M, Carrington M, KewalRamani VN
IL-6 signalling biomarkers in hospitalised patients with moderate to severe SARS-CoV-2 infection in a single centre study in Sweden, 2002-01-29;99(3):1568-73.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
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