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Human DC-SIGN/CD209 PE-conjugated Antibody

Catalog #: FAB161P
11 Citations Datasheet / COA / SDS
Clone 120507 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB161P-100
FAB161P-025
Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry.
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Citations (11)
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Human DC-SIGN/CD209 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC‑SIGN/CD209 on transfected NIH/3T3 cells and on monocyte derived dendritic cells. Does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.
Source
Monoclonal Mouse IgG2B Clone # 120507
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGN/CD209
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DC-SIGN/CD209 antibody in NIH-3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 antibody by Flow Cytometry. View Larger

Detection of DC‑SIGN/CD209 in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN/CD209 by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGN/CD209 was stained with Mouse Anti-Human DC-SIGN/CD209 PE-conjugated Monoclonal Antibody (Catalog # FAB161P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN/CD209

Human DC-SIGN (dendritic cell-specific ICAM-3 grabbing nonintegrin; also known as CD209) is a member of the chromosome 19 C-type lectin family that includes DC-SIGN, DC-SIGN-related protein, CD23 and LSECtin (1). DC-SIGN was initially reported to be a 46 kDa, 404 amino acid (aa) type II transmembrane protein that contained a 40 aa cytoplasmic N-terminus, a 21 aa transmembrane segment, and a 343 aa extracellular C-terminus (2). The extracellular region contains a distal, 115 aa Ca++-dependent carbohydrate-binding lectin domain and a membrane-proximal linker segment that is composed of seven 23 aa repeats (2, 3). The lectin domain is believed to preferably bind mannose, either within the context of ICAM-3 (on T cells) or ICAM-2 (on endothelial cells) (2, 4, 5). DC-SIGN expression appears to be limited to dendritic cells (DC) and macrophages (6), and DC interaction with the ICAMs both aids DC cell trafficking and immunological synapse formation (7). Since the original report on DC-SIGN, multiple splice forms have been discovered, generating both membrane-bound and soluble forms (3). There are eight type A isoforms, all of which begin with the same 15 aa of exon 1a. Four contain the transmembrane region of exon II, and four do not (i.e., are soluble). Among these eight type A isoforms, only three retain the entire 343 aa found in the full length form described in reference #2 (the full length form is referred to as type I mDC-SIGN1A) (3). Five additional isoforms utilize an alternate start site, and these are referred to as type B isoforms. These all show a 35 aa cytoplasmic domain. One also has a transmembrane segment; four do not. Two of the five contain full, unspliced extracellular regions (3). All of this suggests enormous complexity in DC-SIGN biology. DC-SIGN is not well conserved across species. Human and mouse show little overall aa identity. In the lectin domain, however, human DC-SIGN shares 68% aa identity with mouse DC-SIGN (8). Human and rhesus monkey DC-SIGN share 91% aa identity over the entire extracellular region (8). A detailed description of the additional properties of this monoclonal antibody (MAB161) have been published (9, 10).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  3. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.
  4. Su, S.V. et al. (2004) J. Biol. Chem. 279:19122.
  5. Cambi, A. et al. (2005) Cell. Microbiol. 7:481.
  6. Serrano-Gomez, D. et al. (2004) J. Immunol. 173:5635.
  7. Geijtenbeek, T.B.H. and Y. van Kooyk (2003) Curr. Top. Microbiol. Immunol. 276:32.
  8. Baribaud, F. et al. (2001) J. Virol. 75:10281.
  9. Wu, L. et al. (2002) J. Virol. 76:5905.
  10. Baribaud, F. et al. (2002) J. Virol.76:9135.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

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Citations for Human DC-SIGN/CD209 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Elevated glycolytic metabolism of monocytes limits the generation of HIF1A-driven migratory dendritic cells in tuberculosis
    Authors: Maio, M;Barros, J;Joly, M;Vahlas, Z;Marín Franco, JL;Genoula, M;Monard, SC;Vecchione, MB;Fuentes, F;Gonzalez Polo, V;Quiroga, MF;Vermeulen, M;Vu Manh, TP;Argüello, RJ;Inwentarz, S;Musella, R;Ciallella, L;González Montaner, P;Palmero, D;Lugo Villarino, G;Sasiain, MDC;Neyrolles, O;Vérollet, C;Balboa, L;
    eLife
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Early Colorectal Responses to HIV-1 and Modulation by Antiretroviral Drugs
    Authors: C Herrera, MD McRaven, KG Laing, J Dennis, TJ Hope, RJ Shattock
    Vaccines, 2021-03-08;9(3):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Flow Cytometry
  3. Fatty acid oxidation of alternatively activated macrophages prevents foam cell formation, but Mycobacterium tuberculosis counteracts this process via HIF-1&alpha activation
    Authors: M Genoula, JL Marín Fran, M Maio, B Dolotowicz, M Ferreyra, MA Milillo, R Mascarau, EJ Moraña, D Palmero, M Matteo, F Fuentes, B López, P Barrionuev, O Neyrolles, C Cougoule, G Lugo-Villa, C Vérollet, MDC Sasiain, L Balboa
    PLoS Pathog, 2020-10-01;16(10):e1008929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIV
    Authors: JS Johnson, N De Veaux, AW Rives, X Lahaye, SY Lucas, BP Perot, M Luka, V Garcia-Par, LM Amon, A Watters, G Abdessalem, A Aderem, N Manel, DR Littman, R Bonneau, MM Ménager
    Cell Rep, 2020-01-21;30(3):914-931.e9.
    Species: Human
    Sample Types: Whole Cells
  5. Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection
    Authors: JS Johnson, SY Lucas, LM Amon, S Skelton, R Nazitto, S Carbonetti, DN Sather, DR Littman, A Aderem
    Cell Host Microbe, 2018-03-14;23(3):366-381.e9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NK cell heparanase controls tumor invasion and immune surveillance
    Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
    J. Clin. Invest., 2017-06-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV
    Mucosal Immunol, 2016-08-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
    Sci Rep, 2016-07-07;6(0):29447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Dendritic cell-induced activation of latent HIV-1 provirus in actively proliferating primary T lymphocytes.
    Authors: van der Sluis R, van Montfort T, Pollakis G, Sanders R, Speijer D, Berkhout B, Jeeninga R
    PLoS Pathog, 2013-03-21;9(3):e1003259.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. In vitro characterization of primary SIVsmm isolates belonging to different lineages. In vitro growth on rhesus macaque cells is not predictive for in vivo replication in rhesus macaques.
    Authors: Gautam R, Carter AC, Katz N, Butler IF, Barnes M, Hasegawa A, Ratterree M, Silvestri G, Marx PA, Hirsch VM, Pandrea I, Apetrei C
    Virology, 2007-02-15;362(2):257-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. DC-SIGN is the major Mycobacterium tuberculosis receptor on human dendritic cells.
    Authors: Tailleux L, Schwartz O, Herrmann JL, Pivert E, Jackson M, Amara A, Legres L, Dreher D, Nicod LP, Gluckman JC, Lagrange PH, Gicquel B, Neyrolles O
    J. Exp. Med., 2003-01-06;197(1):121-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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