Human DDR2 Biotinylated Antibody

Catalog #: BAF2538 Datasheet / COA / SDS

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BAF2538

All DDR2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DDR2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DDR2 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human DDR1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DDR2
Gln24-Arg399
Accession # Q16832

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human DDR2 (Catalog # 2538-DR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DDR2

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3-5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10-12).

References

Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
Karn, T. et al. (1993) Oncogene 8:3433.
Vogel, W. et al. (1997) Mol. Cell 1:13.
Leitinger, B. (2003) J. Biol. Chem. 278:16761.
Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
Xu, L. et al. (2005) J. Biol. Chem. 280:548.
Wang, J. et al. (2002) J. Autoimmun. 19:161.
Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Long Name

Discoidin Domain Receptor 2

Entrez Gene IDs

4921 (Human); 18214 (Mouse)

Alternate Names

CD167 antigen-like family member B; CD167b antigen; DDR2; Discoidin domain receptor 2; discoidin domain receptor family, member 2; discoidin domain receptor tyrosine kinase 2; discoidin domain-containing receptor 2; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; migration-inducing gene 16 protein; neurotrophic tyrosine kinase receptor related 3; Neurotrophic tyrosine kinase, receptor-related 3; NTRKR3cell migration-inducing protein 20; Receptor protein-tyrosine kinase TKT; TKT; TKTMIG20a; Trk3; TYRO10; Tyro-10; Tyrosine-protein kinase TYRO10; tyrosylprotein kinase