Human Dishevelled-3 Antibody

Catalog #: AF3754 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3754
AF3754-SP

Detection of Human and Mouse Dishevelled-3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dishevelled-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Dishevelled-3
His545-Gly654
Accession # Q92997

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human liver cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Mouse Dishevelled-3 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dishevelled-3

Dishevelled-3 is one of three mammalian multi-moduled Dishevelled proteins that have important roles in the canonical and non-canonical Wnt pathways. Human Dishevelled-3 is a 716 amino acid residue protein with an N-terminal DIX domain, a central PZD domain, and a DEP domain. Over the region used as immunogen, human Dishevelled-3 shares 99% amino acid sequence homology with mouse and canine Dishevelled-3.

Entrez Gene IDs

1857 (Human); 13544 (Mouse); 303811 (Rat)

Alternate Names

dishevelled 3 (homologous to Drosophila dsh); dishevelled, dsh homolog 3 (Drosophila); Dishevelled3; Dishevelled-3; DSH homolog 3; DVL3; KIAA0208dishevelled-3; segment polarity protein dishevelled homolog DVL-3