Human DLL1 ELISA Kit - Quantikine

Catalog #: DLL10 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DLL10

Control Products Available

QC77: Quantikine Immunoassay Control Set 730 for Human DLL1

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human DLL1 Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)

Sensitivity

17.4 pg/mL

Assay Range

78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)

Specificity

Natural and recombinant human DLL10

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human DLL1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DLL1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human DLL1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human DLL1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DLL1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	638	1468	2934	592	1340	2714
Standard Deviation	16	38.1	76.1	42.4	85.7	189
CV%	2.5	2.6	2.6	7.2	6.4	7

Recovery

The recovery of DLL1 spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	96-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DLL1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human DLL1 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DLL1

DLL1 (Delta-like protein 1) is a transmembrane glycoprotein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription.

Long Name:

Delta-like 1

Entrez Gene IDs:

28514 (Human); 13388 (Mouse); 84010 (Rat)

Alternate Names:

delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

100 µL Assay Diluent

Add 100 µL of Assay Diluent to each well.

50 µL Standard, Control, or Sample

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

200 µL Conjugate

Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.