Home / DNAM-1/CD226 / Human DNAM-1/CD226
Antibody

Human DNAM-1/CD226
Antibody

Catalog #: MAB666
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB666-SP
MAB666-100
MAB666-500
Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DNAM-1/CD226
Antibody Summary

Species Reactivity
Human
Specificity
Detects human DNAM-1/CD226 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 102511
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DNAM-1/CD226
Glu19-Asn247 (predicted)
Accession # Q15762
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize DNAM-1/CD226-mediated adhesion of the COLO 205 human colorectal adenocarcinoma cell line. Cosman, D. et al. (1997) Immunity 7:273. The Neutralization Dose (ND50) is typically 0.1‑0.6 µg/mL in the presence of 20 µg/mL Recombinant Human DNAM-1/CD226 Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of DNAM-1/CD226 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of DNAM-1/CD226 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Neutralization Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/CD226 Antibody. View Larger

Cell Adhesion Mediated by DNAM-1/CD226 and Neutral-ization by Human DNAM-1/CD226 Antibody. Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666). The ND50 is typically 0.1-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DNAM-1/CD226

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins (1, 2). Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112 (3, 4). Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells (2‑6), dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts (7‑10). Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung (11). In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation (6, 9, 12‑14).

References
  1. Fuchs, A. and M. Colonna (2006) Semin. Cancer Biol. 16:359.
  2. Shibuya, A. et al. (1996) Immunity 4:573.
  3. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  4. Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
  5. Dardalhon, V. et al. (2005) J. Immunol. 175:1558.
  6. Bachelet, I. et al. (2006) J. Biol. Chem. 281:27190.
  7. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  8. Kakehi, S. et al. (2007) Mol. Cell. Biochem. 301:209.
  9. Kojima, H. et al. (2003) J. Biol. Chem. 278:36748.
  10. Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
  11. Morimoto, K. et al. (2007) Oncogene July 16 epub.
  12. Shibuya, K. et al. (1999) Immunity 11:615.
  13. Shibuya, K. et al. (2003) J. Exp. Med. 198:1829.
  14. Shibuya, A. et al. (1998) J. Immunol. 166:1671.
Long Name
DNAX Accessory Molecule 1
Entrez Gene IDs
10666 (Human); 225825 (Mouse); 307199 (Rat); 102117316 (Cynomolgus Monkey)
Alternate Names
CD226 antigenplatelet and T cell activation antigen 1; CD226 molecule; CD226; DNAM1; DNAM-1; DNAM1adhesion glycoprotein; DNAM-1DNAX accessory molecule-1; DNAX accessory molecule 1; PTA1; T lineage-specific activation antigen 1 antigen; TLiSA1

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Citations for Human DNAM-1/CD226
Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The NK�cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells
    Authors: S Sen Santar, DJ Lee, Â Crespo, JJ Hu, C Walker, X Ma, Y Zhang, S Chowdhury, KF Meza-Sosa, M Lewandrows, H Zhang, M Rowe, A McClelland, H Wu, C Junqueira, J Lieberman
    Nature, 2023-04-05;616(7956):348-356.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. DNAM-1/CD226 is functionally expressed on acute myeloid leukemia (AML) cells and is associated with favorable prognosis
    Authors: A Chashchina, M Märklin, C Hinterleit, HR Salih, JS Heitmann, B Klimovich
    Scientific Reports, 2021-09-09;11(1):18012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation
    Authors: M Najar, M Fayyad-Kaz, N Meuleman, D Bron, H Fayyad-Kaz, L Lagneaux
    J Cell Commun Signal, 2018-01-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Impaired NK cell recognition of vemurafenib-treated melanoma cells is overcome by simultaneous application of histone deacetylase inhibitors
    Authors: S López-Cobo, N Pieper, C Campos-Sil, EM García-Cue, HT Reyburn, A Paschen, M Valés-Góme
    Oncoimmunology, 2017-11-06;7(2):e1392426.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The Repeated Administration of Resveratrol Has Measurable Effects on Circulating T-Cell Subsets in Humans
    Authors: JL Espinoza, LQ Trung, PT Inaoka, K Yamada, DT An, S Mizuno, S Nakao, A Takami
    Oxid Med Cell Longev, 2017-05-04;2017(0):6781872.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. CD155 on HIV-Infected Cells Is Not Modulated by HIV-1 Vpu and Nef but Synergizes with NKG2D Ligands to Trigger NK Cell Lysis of Autologous Primary HIV-Infected Cells.
    Authors: Davis Z, Sowrirajan B, Cogswell A, Ward J, Planelles V, Barker E
    AIDS Res Hum Retroviruses, 2016-07-20;33(2):93-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.
    Authors: Huijskens M, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers B, Tilanus M, Bos G, Wieten L, Germeraad W
    Cytotherapy, 2015-03-05;17(5):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Killing of myeloid APCs via HLA class I, CD2 and CD226 defines a novel mechanism of suppression by human Tr1 cells.
    Authors: Magnani CF, Alberigo G, Bacchetta R, Serafini G, Andreani M, Roncarolo MG, Gregori S
    Eur. J. Immunol., 2011-05-13;41(6):1652-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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