Home / Dopamine D1R/DRD1 / Human Dopamine D1R/DRD1 Antibody

Human Dopamine D1R/DRD1 Antibody

Catalog #: MAB8276
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB8276
MAB8276-SP
Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dopamine D1R/DRD1 Antibody Summary

Species Reactivity
Human
Specificity
Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 887438
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Dopamine D1 R/DRD1 antibody in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP antibody by Flow Cytometry. View Larger

Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP was stained with either (A) Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Immunohistochemistry Dopamine D1 R/DRD1 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Dopamine D1 R/DRD1 in Human Brain. Dopamine D1 R/DRD1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopamine D1R/DRD1

Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.

Long Name
Dopamine D1 Receptor
Entrez Gene IDs
1812 (Human); 13488 (Mouse); 24316 (Rat)
Alternate Names
D(1A) dopamine receptor; D1DR; DADR; Dopamine D1 R; Dopamine D1 receptor; Dopamine D1R; dopamine receptor D1; DRD1; DRD1A

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human Dopamine D1R/DRD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dopamine receptors D1 and D2 show prognostic significance and potential therapeutic applications for endometrial cancer patients
    Authors: Español, P;Rovira, R;Caruana, P;Luna-Guibourg, R;Soler, C;Teixeira, N;Rodríguez, F;Gallardo, A;Edwards, M;Porta, O;Gámez, M;Sánchez, O;Llurba, E;Corchero, JL;Céspedes, MV;
    Gynecologic oncology
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Dopamine D1R/DRD1 Antibody

There are currently no reviews for this product. Be the first to review Human Dopamine D1R/DRD1 Antibody and earn rewards!

Have you used Human Dopamine D1R/DRD1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review