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Human DPPIV/CD26 ELISA Kit - Quantikine

Catalog #: DC260B
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Human DPPIV/CD26 ELISA Standard Curve
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Citations (8)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human DPPIV/CD26 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)
Sensitivity
0.072 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human DPPIV.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human DPPIV/CD26 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DPPIV in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human DPPIV, and antibodies raised against the recombinant factor. Results obtained using natural human DPPIV showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human DPPIV.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 2.16 8.15 14.1 2.22 8.25 14.6
Standard Deviation 0.105 0.28 0.916 0.14 0.695 1.18
CV% 4.9 3.4 6.5 6.3 8.4 8.1

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 2.32 8.78 15.8 2.43 8.53 15.9
Standard Deviation 0.142 0.354 1.15 0.207 0.777 1.3
CV% 6.1 4 7.3 8.5 9.1 8.2

Recovery

The recovery of human DPPIV spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 87-113
Urine (n=4) 101 88-113

Linearity

To assess linearity of the assay, samples containing and/or spiked with high concentrations of human DPPIV were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human DPPIV/CD26 Simple Plex Assay Linearity
Human DPPIV/CD26 ELISA Linearity

Scientific Data

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Human DPPIV/CD26 ELISA Standard Curve

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Human DPPIV/CD26 ELISA Standard Curve

N/A Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve View Larger

Human DPPIV/CD26 ELISA Cell Culture Supernate/Saliva/Urine Standard Curve

N/A Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve View Larger

Human DPPIV/CD26 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DPPIV/CD26

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Long Name:
Dipeptidyl-peptidase IV
Entrez Gene IDs:
1803 (Human); 13482 (Mouse); 397492 (Porcine); 102133935 (Cynomolgus Monkey)
Alternate Names:
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human DPPIV/CD26 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Low levels of soluble DPP4 among Saudis may have constituted a risk factor for MERS endemicity
    Authors: KR Alkharsah, SA Aljaroodi, JU Rahman, AN Alnafie, R Al Dossary, RY Aljindan, AM Alnimr, J Hussen
    PLoS ONE, 2022-04-12;17(4):e0266603.
    Species: Human
    Sample Types: Plasma
  2. Adipokines and inflammatory markers in elderly subjects with high risk of type 2 diabetes and cardiovascular disease
    Authors: T Saukkonen, SJ Mutt, J Jokelainen, AM Saukkonen, GS Raza, T Karhu, P Härkönen, J Eckel, KH Herzig, U Rajala, S Keinänen-K
    Sci Rep, 2018-08-24;8(1):12816.
    Species: Human
    Sample Types: Plasma
  3. Reduction of soluble dipeptidyl peptidase 4 levels in plasma of patients infected with Middle East respiratory syndrome coronavirus
    Authors: KS Inn, Y Kim, A Aigerim, U Park, ES Hwang, MS Choi, YS Kim, NH Cho
    Virology, 2018-03-26;518(0):324-327.
    Species: Human
    Sample Types: Sputum
  4. Correction of vitamin D deficiency facilitated suppression of IP-10 and DPP IV levels in patients with chronic hepatitis C: A randomised double-blinded, placebo-control trial
    Authors: P Komolmit, K Charoensuk, K Thanapirom, S Suksawatam, P Thaimai, C Chirathawo, Y Poovorawan
    PLoS ONE, 2017-04-04;12(4):e0174608.
    Species: Human
    Sample Types: Serum
  5. Dipeptidyl peptidase-IV in chronic heart failure with reduced ejection fraction
    Authors: P Lourenço, S Silva, F Cunha, J Pereira, A Ribeiro, N Silva, JT Guimarães, JP Araújo, P Bettencour
    Int. J. Cardiol, 2017-03-11;0(0):.
    Species: Human
    Sample Types: Serum
  6. Adiponectin, chemerin, cytokines, and dipeptidyl peptidase 4 are released from human adipose tissue in a depot-dependent manner: an in vitro system including human serum albumin.
    Authors: Svensson H, Oden B, Eden S, Lonn M
    BMC Endocr Disord, 2014-01-22;14(0):7.
    Species: Human
    Sample Types: Tissue Homogenates
  7. Early and late effects of the DPP-4 inhibitor vildagliptin in a rat model of post-myocardial infarction heart failure.
    Authors: Yin M, Sillje HH, Meissner M, van Gilst WH, de Boer RA
    Cardiovasc Diabetol, 2011-09-28;10(0):85.
    Species: Human
    Sample Types: Plasma
  8. Serum level of CD26 predicts time to first treatment in early B-chronic lymphocytic leukemia.
    Authors: Molica S, Digiesi G, Mirabelli R, Cutrona G, Antenucci A, Molica M, Giannarelli D, Sperduti I, Morabito F, Neri A, Baldini L, Ferrarini M
    Eur. J. Haematol., 2009-05-02;83(0):208-14.
    Species: Human
    Sample Types: Serum

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