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Human Tyro3/Dtk PE-conjugated Antibody

Catalog #: FAB859P
7 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB859P
Detection of Dtk in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tyro3/Dtk PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Dtk in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity or interference with recombinant mouse Dtk, recombinant human (rh) Axl, or rhMer is observed.
Source
Monoclonal Mouse IgG1 Clone # 96201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dtk
Ala41-Ser428 (predicted)
Accession # Q06418
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Dtk antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Dtk in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Dtk PE-conjugated Monoclonal Antibody (Catalog # FAB859P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Tyro3/Dtk

Dtk (also known as Sky, Tyro3, Rse, Brt), Axl (also known as Ufo, Ark) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor Protein S. The binding affinities for Gas6 are in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

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Citations for Human Tyro3/Dtk PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
    Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
    bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK
    Authors: J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su
    Arthritis Research & Therapy, 2020-09-21;22(1):221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Protein S and Gas6 induce efferocytosis of HIV-1-infected cells
    Authors: BA Chua, JA Ngo, K Situ, CM Ramirez, H Nakano, K Morizono
    Virology, 2018-01-02;515(0):176-190.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Phosphatidylserine Sensing by TAM Receptors Regulates AKT-Dependent Chemoresistance and PD-L1 Expression.
    Authors: Kasikara C, Kumar S, Kimani S, Tsou W, Geng K, Davra V, Sriram G, Devoe C, Nguyen K, Antes A, Krantz A, Rymarczyk G, Wilczynski A, Empig C, Freimark B, Gray M, Schlunegger K, Hutchins J, Kotenko S, Birge R
    Mol Cancer Res, 2017-02-09;15(6):753-764.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Axl is required for TGF-?2-induced dormancy of prostate cancer cells in the bone marrow
    Sci Rep, 2016-11-07;6(0):36520.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages.
    Authors: Zheng S, Hedl M, Abraham C
    J Immunol, 2015-01-07;194(4):1928-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.
    Authors: Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA
    J. Thromb. Haemost., 2005-02-23;3(4):733-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Tyro3/Dtk PE-conjugated Antibody

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Human Tyro3/Dtk PE-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

the antibody was used to stain potential zika virus receptor Tyro3/Dtk in PMID: 29262327. it works very well.

Human Dtk PE-conjugated Antibody
By Anonymous on 09/10/2016
Application: Flow Sample Tested: iDC human immature myeloid dendritic cells Species: Human
Flow Cytometry Human Dtk PE-conjugated Antibody FAB859P