Home / E-Selectin/CD62E / Human E-Selectin/CD62E Antibody

Human E-Selectin/CD62E Antibody

Catalog #: BBA16
54 Citations 3 Reviews Datasheet / COA / SDS
Clone BBIG-E4 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
BBA16
Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.
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Product Details
Citations (54)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Selectin/CD62E Antibody Summary

Species Reactivity
Human
Specificity
Detects E‑Selectin/CD62E in ELISAs and Western blots. In immunofluorescence experiments, does not stain COS cells transfected with cDNA for human ICAM-1, PECAM‑1, L-Selectin, P-Selectin, or VCAM-1.
Source
Monoclonal Mouse IgG1 Clone # BBIG-E4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
Human umbilical cord endothelial cells (HUVECs) cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (Catalog # 210-TA)
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
 
Immunocytochemistry
8-25 µg/mL
See below

Human E-Selectin/CD62E Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-10 µg/mL 

Use in combination with:

Detection Reagent: Human E‑Selectin/CD62E Biotinylated Antibody (Catalog # BBA8)

Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry. Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry E-Selectin/CD62E antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

E-Selectin/CD62E in HUVEC Human Cells. E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells activated with TNF-a (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin/ CD62E Monoclonal Antibody (Catalog # BBA16) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry E‑Selectin/CD62E antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

E‑Selectin/CD62E in Human Brain. E-Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human brain (occipital cortex) using 10 µg/mL Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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Citations for Human E-Selectin/CD62E Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

54 Citations: Showing 1 - 10
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  1. Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8
    Authors: T Pirker, EM Pferschy-W, E Bampali, V Bochkov, R Bauer
    Journal of ethnopharmacology, 2023-03-02;0(0):116328.
    Species: Human
    Sample Types: Cell culture supernate
    Applications: BBA16
  2. Stabilization of Endothelial Receptor Arrays by a Polarized Spectrin Cytoskeleton Facilitates Rolling and Adhesion of Leukocytes
    Authors: S Mylvaganam, M Riedl, A Vega, RF Collins, K Jaqaman, S Grinstein, SA Freeman
    Cell Rep, 2020-06-23;31(12):107798.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. A Symphytum officinale Root Extract Exerts Anti-inflammatory Properties by Affecting Two Distinct Steps of NF-?B Signaling
    Authors: J Seigner, M Junker-Sam, A Plaza, G D'Urso, M Masullo, S Piacente, YM Holper-Sch, R de Martin
    Front Pharmacol, 2019-04-26;10(0):289.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA - Cell Based
  4. Pinocembrin protects endothelial cells from oxidized LDL-induced injury
    Authors: Q Su, Y Sun, Z Ye, H Yang, B Kong, L Li
    Cytokine, 2018-06-18;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Bordetella pertussis adenylate cyclase toxin disrupts functional integrity of bronchial epithelial layers
    Authors: S Hasan, NN Kulkarni, A Asbjarnars, I Linhartova, R Osicka, P Sebo, GH Gudmundsso
    Infect. Immun., 2018-02-20;0(0):.
  6. A modular platform for targeted RNAi therapeutics
    Authors: R Kedmi, N Veiga, S Ramishetti, M Goldsmith, D Rosenblum, N Dammes, I Hazan-Hale, L Nahary, S Leviatan-B, M Harlev, M Behlke, I Benhar, J Lieberman, D Peer
    Nat Nanotechnol, 2018-01-29;0(0):.
  7. Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression
    Authors: C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al
    Sci Rep, 2018-01-11;8(1):420.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer
    Authors: BV Howley, LA Link, S Grelet, M El-Sabban, PH Howe
    Oncogene, 2017-12-18;0(0):.
  9. Analysis of neuronal phosphoproteome reveals PINK1 regulation of BAD function and cell death
    Authors: H Wan, B Tang, X Liao, Q Zeng, Z Zhang, L Liao
    Cell Death Differ., 2017-12-12;0(0):.
  10. Erythropoietin signaling increases neurogenesis and oligodendrogenesis of endogenous neural stem cells following spinal cord injury both in�vivo and in�vitro
    Authors: H Zhang, X Fang, D Huang, Q Luo, M Zheng, K Wang, L Cao, Z Yin
    Mol Med Rep, 2017-10-25;0(0):.
  11. RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo
    Authors: L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster
    Mol. Ther., 2017-10-13;0(0):.
  12. Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
    Authors: Tra M T Bui
    Reprod. Fertil. Dev, 2017-09-01;0(0):.
  13. The Abl-1 kinase is dispensable for NK cell inhibitory signaling and is not involved in murine NK cell education
    Authors: S Ganesan, TL Thanh, N Kadri, BJ Chambers, S Meinke, P Brodin, E Vivier, DM Wetzel, AJ Koleske, P Höglund
    Scand. J. Immunol., 2017-09-01;0(0):.
  14. Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
    Authors: K Yan, Y Shen
    Mol Med Rep, 2017-07-28;0(0):.
  15. Anti-CD20 monoclonal antibody combined with adenovirus vector-mediated IL-10 regulates spleen CD4+/CD8+ T�cells and T-bet/GATA-3 expression in NOD mice
    Authors: A Tang, C Li, Z Chen, T Li
    Mol Med Rep, 2017-07-28;0(0):.
  16. Heme oxygenase-1 directly binds STAT3 to control the generation of pathogenic Th17 cells during neutrophilic airway inflammation
    Authors: X Lin, J Lv, J Lv, C Di, Y Zhang, T Zhou, J Liu, Z Xia
    Allergy, 2017-07-03;0(0):.
  17. Blockade of interleukin-6 receptor in the periphery promotes rapid and sustained antidepressant actions: a possible role of gut-microbiota-brain axis
    Authors: JC Zhang, W Yao, C Dong, C Yang, Q Ren, M Ma, K Hashimoto
    Transl Psychiatry, 2017-05-30;7(5):e1138.
  18. Protection of Dexmedetomidine Against Ischemia/Reperfusion-Induced Apoptotic Insults to Neuronal Cells Occurs via an Intrinsic Mitochondria-Dependent Pathway
    Authors: Gong-Jhe Wu
    J. Cell. Biochem, 2017-05-16;0(0):.
  19. Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture
    Authors: Xuejun Cheng
    Dev Neurobiol, 2017-03-23;0(0):.
  20. Purinergic receptor transactivation by the ?2-adrenergic receptor increases intracellular Ca(2+) in non-excitable cells
    Authors: W Stallaert, ET van der We, AM Schonegge, B Plouffe, M Hogue, V Lukashova, A Inoue, S Ishida, J Aoki, C Le Gouill, M Bouvier
    Mol. Pharmacol, 2017-03-09;0(0):.
  21. Localized delivery of curcumin from injectable gelatin/Thai silk fibroin microspheres for anti-inflammatory treatment of osteoarthritis in a rat model
    Authors: J Ratanavara, K Soontornvi, S Shuangshot, S Shuangshot, S Damrongsak
    Inflammopharmacology, 2017-03-01;0(0):.
  22. Amine oxidase activity regulates the development of pulmonary fibrosis
    Authors: F Marttila-I, K Elima, K Auvinen, TZ Veres, P Rantakari, C Weston, M Miyasaka, D Adams, S Jalkanen, M Salmi
    FASEB J, 2017-03-01;0(0):.
  23. Hepatic inflammation-fibrosis-cancer axis in the rat hepatocellular carcinoma induced by diethylnitrosamine
    Authors: YF Ding, ZH Wu, YJ Wei, L Shu, YR Peng
    J. Cancer Res. Clin. Oncol, 2017-02-25;0(0):.
  24. AGER gene polymorphisms and soluble receptor for advanced glycation end product in patients with idiopathic pulmonary fibrosis
    Authors: K Yamaguchi, H Iwamoto, Y Horimasu, S Ohshimo, K Fujitaka, H Hamada, W Mazur, N Kohno, N Hattori
    Respirology, 2017-02-14;0(0):.
  25. Phenotypic and Functional Alterations of Hematopoietic Stem and Progenitor Cells in an In Vitro Leukemia-Induced Microenvironment
    Authors: JP Vernot, X Bonilla, V Rodriguez-, NP Vanegas
    Int J Mol Sci, 2017-02-14;18(2):.
  26. Soluble ST2 Predicts 1-Year Outcome in Patients Undergoing Transcatheter Aortic Valve Implantation (TAVI)
    Authors: Bernhard Wernly
    Eur. J. Clin. Invest, 2017-02-01;0(0):.
  27. 3D co-culture model to analyze the crosstalk between endothelial and smooth muscle cells
    Authors: Minu Karthika Ganesan
    Tissue Eng Part C Methods, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: ICC
  28. INF-? encoding plasmid administration triggers bone loss and disrupts bone marrow microenvironment
    Authors: Dimitrios Agas
    J. Endocrinol, 2016-12-01;0(0):.
  29. IL-17RB(+) granulocytes are associated with airflow obstruction in asthma
    Authors: Lin Li
    Ann. Allergy Asthma Immunol, 2016-12-01;117(6):674-679.
  30. Characterization of guinea pig T cell responses elicited after EP-assisted delivery of DNA vaccines to the skin
    Authors: Trevor R F Smith
    Vaccine, 2016-11-25;35(1):61-70.
  31. Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats
    Authors: Zhonghao Liu
    Can J Diabetes, 2016-11-23;0(0):.
  32. BMP4/Id2 signaling pathway is a novel therapeutic target for late outgrowth endothelial progenitor cell-mediated endothelial injury repair
    Authors: Jun Tao
    Int. J. Cardiol., 2016-11-09;228(0):796-804.
  33. Proliferation, differentiation and migration of SCA1(-)/CD31(-) cardiac side population cells in vitro and in vivo
    Authors: Simon X Liang
    Int. J. Cardiol., 2016-11-09;0(0):.
  34. Role of Epigenetic Modification and Immunomodulation in a Murine Prostate Cancer Model
    Authors: Georgi Guruli
    Prostate, 2016-11-08;0(0):.
  35. Neutrophil gelatinase-associated lipocalin predicts myocardial dysfunction and mortality in severe sepsis and septic shock
    Authors: Xiaoye Yan
    Int. J. Cardiol., 2016-11-03;0(0):.
  36. Endothelin-1 Overexpression Exaggerates Diabetes-Induced Endothelial Dysfunction by Altering Oxidative Stress
    Am J Hypertens, 2016-11-01;0(0):.
  37. Impact of exercise training associated to pyridostigmine treatment on autonomic function and inflammatory profile after myocardial infarction in rats
    Authors: Bruno Rodrigues
    Int. J. Cardiol., 2016-10-26;0(0):.
  38. Cell surface-anchored syndecan-1 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis
    J Cell Mol Med, 2016-08-25;0(0):.
  39. Plasma membrane TLR activation increases bacterial uptake but abrogates endosomal Lactobacillus acidophilus induction of Interferon-B
    Immunology, 2016-08-24;0(0):.
  40. C13orf31 (FAMIN) is a central regulator of immunometabolic function
    Nat Immunol, 2016-08-01;0(0):.
  41. Critical role of fatty acid metabolism in ILC2-mediated barrier protection during malnutrition and helminth infection.
    Authors: Christoph Wilhelm, Oliver J Harrison, Vanessa Schmitt, Martin Pelletier, Sean P Spencer, Joseph F Urban, Michelle Ploch, Thirumalai R Ramalinga, Richard M Siegel, Yasmine Belkaid
    The Journal of Experimental Medicine, 2016-07-18;0(0):1540-9538.
  42. Activation of IFN/STAT1 signalling predicts response to chemotherapy in oestrogen receptor-negative breast cancer
    Authors: ME Legrier, I BiŠche, J Gaston, A Beurdeley, V Yvonnet, O D‚as, A Thuleau, S Chƒteau-Jo, JL Servely, S Vacher, M Lassalle, S Depil, GC Tucker, JJ Fontaine, MF Poupon, S Roman-Roma, JG Judde, D Decaudin, S Cairo, E Marangoni
    Br. J. Cancer, 2015-12-22;114(2):177-87.
  43. TIGIT expression levels on human NK cells correlate with functional heterogeneity among healthy individuals.
    Authors: Wang F, Hou H, Wu S, Tang Q, Liu W, Huang M, Yin B, Huang J, Mao L, Lu Y, Sun Z
    Eur J Immunol, 2015-09-07;45(10):2886-97.
  44. Blocking the adhesion cascade at the premetastatic niche for prevention of breast cancer metastasis.
    Authors: Kang S, Hasan N, Mann A, Zheng W, Zhao L, Morris L, Zhu W, Zhao Y, Suh K, Dooley W, Volk D, Gorenstein D, Cristofanilli M, Rui H, Tanaka T
    Mol Ther, 2015-03-27;23(6):1044-54.
  45. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
    Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
    PLoS ONE, 2013-04-01;8(4):e60823.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  46. Endothelial cells of human colorectal cancer and healthy colon reveal phenotypic differences in culture.
    Authors: Schellerer VS, Croner RS, Weinlander K, Hohenberger W, Sturzl M, Naschberger E
    Lab. Invest., 2007-09-17;87(11):1159-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  47. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006-11-20;203(12):2763-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  48. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006-08-01;108(3):928-35.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  49. A role for the L-selectin adhesion system in mediating cytotrophoblast emigration from the placenta.
    Authors: Prakobphol A, Genbacev O, Gormley M, Kapidzic M, Fisher SJ
    Dev. Biol., 2006-06-15;298(1):107-17.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
    Authors: Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
    Blood, 2005-11-29;107(6):2252-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  51. The role of endothelial cell reactive antibodies in peripheral arterial disease.
    Authors: Armitage JD, Lindsey NJ, Homer-Vanniasinkam S
    Eur J Vasc Endovasc Surg, 2005-10-14;31(2):170-5.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  52. Differential effects of orbital and laminar shear stress on endothelial cells.
    Authors: Frattini J, Kudo FA
    J. Vasc. Surg., 2005-05-01;41(5):869-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  53. Persistent cytomegalovirus infection is associated with increased expression of TGF-beta1, PDGF-AA and ICAM-1 and arterial intimal thickening in kidney allografts.
    Authors: Helantera I, Loginov R, Koskinen P, Tornroth T, Gronhagen-Riska C, Lautenschlager I
    Nephrol. Dial. Transplant., 2005-02-16;20(4):790-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  54. Endothelium properties of a tissue-engineered blood vessel for small-diameter vascular reconstruction.
    Authors: Remy-Zolghadri M, Laganiere J, Oligny JF, Germain L, Auger FA
    J. Vasc. Surg., 2004-03-01;39(3):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human E-Selectin/CD62E Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human E-Selectin/CD62E Antibody
By Anonymous on 08/18/2021
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
Immunocytochemistry/Immunofluorescence Human E-Selectin/CD62E Antibody BBA16
Human E-Selectin/CD62E Antibody
By Anonymous on 08/11/2021
Application: IHC Sample Tested: Thyroid carcinoma Species: Human
Immunohistochemistry Human E-Selectin/CD62E Antibody BBA16
Human E-Selectin/CD62E Antibody
By Michelle Chen on 04/26/2017
Application: ICC/IF Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human

HUVECs were stimulated with LPS for activation