Human E-Selectin/CD62E Biotinylated Antibody

Catalog #: BBA8 Datasheet / COA / SDS

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Human E-Selectin/CD62E Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

This antibody stains COS cells transfected with cDNA for human E-Selectin/CD62E in immunofluorescence experiments. This antibody does not stain COS cells transfected with cDNA for human ICAM-1, PECAM-1 or VCAM-1.

Source

Monoclonal Mouse IgG₁ Clone # BBIG-E5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
under non-reducing conditions only

Immunocytochemistry

8-25 µg/mL

See below

Human E-Selectin/CD62E Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human E‑Selectin/CD62E Antibody (Catalog # BBA2)

Capture Reagent: Human E‑Selectin/CD62E Antibody (Catalog # BBA16)

Standard: Recombinant Human E-Selectin/CD62E Protein, CF (Catalog # ADP1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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E-Selectin/CD62E in HUVEC Cells. E-Selectin/CD62E was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) activated with TNF alpha using Mouse Anti-Human E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # BBA8) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: E-Selectin/CD62E

E-Selectin, also known as Endothelial Leukocyte Adhesion Molecule 1 (ELAM-1), and Leukocyte-Endothelial Cell Adhesion Molecule 2 (LECAM-2), has been designated CD62E. It is a cell-surface glycoprotein expressed by activated endothelium and it mediates the adhesion of blood neutrophils.

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Alternate Names

CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E