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Human E-Selectin/CD62E Fluorescein-conjugated Antibody

Catalog #: BBA21
8 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA21
Detection of CD62E in HUVECs by Flow Cytometry.
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Product Details
Citations (8)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E-Selectin/CD62E Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human E‑Selectin/CD62E
Source
Monoclonal Mouse IgG1 Clone # BBIG-E5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD62E antibody in HUVECs antibody by Flow Cytometry. View Larger

Detection of CD62E in HUVECs by Flow Cytometry. Human umbilical cord endothelial cells cultured for 4 hours in the presence of 10 ng/mL of rhTNF-a were stained with anti-human CD62E-Fluorescein (R&D Systems, Cat. # BBA21, filled histogram) or with isotype control (R&D Systems, Cat. # IC002F, open histogram).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E) is a 115 kDa type-I membrane glycoprotein expressed only on endothelial cells and only after activation by inflammatory cytokines such as IL-1 beta and TNF-alpha or endotoxin (1 - 4). Expression is transitory reaching a maximum within 6 hours of stimulation and then declining with the generation of a soluble form of E-Selectin (1 - 5). Expression of E-Selectin on cell surfaces facilitates the rolling attachment of leukocytes to the endothelium which is an important step in the extravasation of leukocytes at sites of inflammation (1 - 6). E-Selectin is thought to play a prominent role in inflammatory precesses of the skin (4).

Entrez Gene IDs
6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)
Alternate Names
CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E

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Citations for Human E-Selectin/CD62E Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells
    Authors: D Eckert, F Rapp, AT Tsedeke, J Molendowsk, R Lehn, M Langhans, C Fournier, F Rödel, S Hehlgans
    Cells, 2021-12-27;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Bone Morphogenetic Protein 9 Enhances Lipopolysaccharide-Induced Leukocyte Recruitment to the Vascular Endothelium
    J Immunol, 2016-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The endocannabinoid/endovanilloid N-arachidonoyl dopamine (NADA) and synthetic cannabinoid WIN55,212-2 abate the inflammatory activation of human endothelial cells.
    Authors: Wilhelmsen K, Khakpour S, Tran A, Sheehan K, Schumacher M, Xu F, Hellman J
    J Biol Chem, 2014-03-18;289(19):13079-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TWEAK/Fn14 pathway modulates properties of a human microvascular endothelial cell model of blood brain barrier.
    Authors: Stephan D, Sbai O, Wen J, Couraud P, Putterman C, Khrestchatisky M, Desplat-Jego S
    J Neuroinflammation, 2013-01-15;10(0):9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
    Authors: Huang RB, Eniola-Adefeso O
    PLoS ONE, 2012-02-24;7(2):e31874.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells.
    Authors: Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R
    Atherosclerosis, 2006-03-30;190(1):90-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Angiopoietin 1 and vascular endothelial growth factor modulate human glomerular endothelial cell barrier properties.
    Authors: Satchell SC, Anderson KL, Mathieson PW
    J. Am. Soc. Nephrol., 2004-03-01;15(3):566-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human E-Selectin/CD62E Fluorescein-conjugated Antibody
By Anonymous on 07/07/2017
Application: Flow Sample Tested: Peripheral blood cells Species: Human