Human EDNRB/Endothelin R Type B Antibody

Catalog #: MAB4496 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4496
MAB4496-SP

Detection of Human EDNRB/Endothelin R Type B by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EDNRB/Endothelin R Type B Antibody Summary

Species Reactivity

Human

Specificity

Detects human EDNRB/Endothelin R Type B in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 671917

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human EDNRB/Endothelin R Type B
Accession # P24530

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

1-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human EDNRB/Endothelin R Type B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human EDNRB/Endothelin R Type B. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human EDNRB/ Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EDNRB/Endothelin R Type B at approximately 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of EDNRB in HEK293 Human Cell Line Transfected with Human EDNRB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human EDNRB or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human EDNRB Monoclonal Antibody (Catalog # MAB4496) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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EDNRB/Endothelin R Type B in Human Placenta. EDNRB/Endothelin R Type B was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human EDNRB/Endothelin R Type B Monoclonal Antibody (Catalog # MAB4496) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRB/Endothelin R Type B

EDNRB (Endothelin Receptor Type B) is a 50-55 kDa member of the beta-family of rhodopsin receptors. It binds endothelin 1, 2 and 3, and is found on endothelial cells where it mediates vasodilation. Mature human EDNRB is a 7-transmembrane glycoprotein that is 416 amino acids (aa) in length. It contains a 75 aa N-terminal extracellular region (aa 27-101), and a 44 aa C-terminal cytoplasmic domain. There are three EDNRB variants that affect aa 27-101. One shows a 90 aa N-terminal extension, a second shows the same 90 aa N-terminal substitution coupled with a deletion of aa 268-398, and a third shows proteolytic cleavage between Arg64‑Ser65. Over aa 27-101, human EDNRB shares 67% and 97% aa identity with mouse and canine EDNRB, respectively.

Long Name

Endothelin Receptor Type B

Entrez Gene IDs

1910 (Human); 13618 (Mouse); 50672 (Rat)

Alternate Names

ABCDS; EDNRB; endothelin B receptor; Endothelin receptor non-selective type; endothelin receptor type B; ETB; ET-B; ETBR; ET-BR; ETRB; HSCR; HSCR2; WS4A