Human EGF Antibody

Catalog #: AF236
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF236
AF236-SP

Detection of Recombinant Human, Mouse, and Rat EGF by Western Blot.

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All EGF Antibodies

Product Details

Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EGF Antibody Summary

Species Reactivity

Human

Specificity

Detects human EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant rat EGF and 5% cross-reactivity with recombinant mouse EGF is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human EGF

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.040 - 0.480 µg/mL in the presence of 2 ng/mL Recombinant Human EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Recombinant Human, Mouse, and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry

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EGF in Human Placenta. EGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization

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Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF236). The ND₅₀ is typically 0.040 - 0.480 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

EGF is the prototypic member of a family of growth factors that are characterized by the presence of EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human EGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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ALCAM regulates multiple myeloma chemoresistant side population
Authors: F Wang, Z Dan, H Luo, J Huang, Y Cui, H Ding, J Xu, Z Lin, Y Gao, X Zhai, Y Yang, Y Qu, L Zhang, F Chen, Q Wang, X Wang, Y Feng, T Liu, Q Yi, T Niu, Y Zheng
Cell Death & Disease, 2022-02-10;13(2):136.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2019-05-13;11(5):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
Int J Mol Sci, 2019-01-02;20(1):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
J. Immunol. Methods, 2012-02-17;378(1):72-80.
Species: Human
Sample Types: Serum
Applications: ELISA Development
EGFR signaling is required for TGF-beta 1 mediated COX-2 induction in human bronchial epithelial cells.
Authors: Liu M, Yang SC, Sharma S, Luo J, Cui X, Peebles KA, Huang M, Sato M, Ramirez RD, Shay JW, Minna JD, Dubinett SM
Am. J. Respir. Cell Mol. Biol., 2007-06-28;37(5):578-88.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Epidermal growth factor-like growth factors prevent apoptosis of alcohol-exposed human placental cytotrophoblast cells.
Authors: Wolff GS, Chiang PJ, Smith SM, Romero R, Armant DR
Biol. Reprod., 2007-03-28;77(1):53-60.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells.
Authors: Lee JH, Ryu IH, Kim EK, Lee JE, Hong S, Lee HK
Invest. Ophthalmol. Vis. Sci., 2006-03-01;47(3):864-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Expression of growth factors and growth factor receptor in non-healing and healing ischaemic ulceration.
Authors: Murphy MO, Ghosh J, Fulford P, Khwaja N, Halka AT, Carter A, Turner NJ, Walker MG
Eur J Vasc Endovasc Surg, 2006-01-20;31(5):516-22.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Heparin-binding EGF-like growth factor regulates human extravillous cytotrophoblast development during conversion to the invasive phenotype.
Authors: Leach RE, Kilburn B, Wang J, Liu Z, Romero R, Armant DR
Dev. Biol., 2004-02-15;266(2):223-37.
Species: Human
Sample Types: Whole Cells
Applications: ICC, Neutralization