Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of EGF R/ErbB1 in A431 human epithelial carcinoma cell line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Human Anti-Human EGF R/ErbB1 (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B, filled histogram) or irrelevant biotinylated antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
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EGFR in A431 Human Cell Line. EGFR was detected in unfixed live A431 human epithelial carcinoma cell line using Human Anti-Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9577B) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EGFR
Cetuximab is a biosimilar directed against the extracellular domain of EGFR. The biotinylated antibody can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.
Product Datasheets
Citations for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Regulatory programs of B-cell activation and germinal center reaction allow B-ALL escape from CD19 CAR T-cell therapy
Authors: NG Im, A Guillaumet, M Wal, AJ Rogers, J Frede, CC Havig, J Yang, P Anand, SK Stegmann, JM Waldschmid, N Sotudeh, L Niu, J Voisine, MR Schweiger, C Grassberge, JG Lohr, B Knoechel
Oncogene, 2022-09-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Evaluation of the Targeting and Therapeutic Efficiency of Anti-EGFR Functionalised Nanoparticles in Head and Neck Cancer Cells for Use in NIR-II Optical Window
Authors: T Egnuni, N Ingram, I Mirza, PL Coletta, JR McLaughlan
Pharmaceutics, 2021-10-09;13(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Modeling, optimization, and comparable efficacy of T cell and hematopoietic stem cell gene editing for treating hyper-IgM syndrome
Authors: V Vavassori, E Mercuri, GE Marcovecch, MC Castiello, G Schiroli, L Albano, C Margulies, F Buquicchio, E Fontana, S Beretta, I Merelli, A Cappelleri, PM Rancoita, V Lougaris, A Plebani, M Kanariou, A Lankester, F Ferrua, E Scanziani, C Cotta-Ramu, A Villa, L Naldini, P Genovese
Embo Molecular Medicine, 2021-01-21;0(0):e13545.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
FAQs
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What is the light chain for Human EGFR (Research Grade Cetuximab Biosimilar) Antibody, Catalog #s MAB9577 and FAB9577, Clone # Hu1?
Catalog #s MAB9577 and FAB9577 have a kappa light chain.
Reviews for Human EGFR (Research Grade Cetuximab Biosimilar) Biotinylated Antibody
Average Rating: 4 (Based on 1 Review)
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Red histogram: HEK293 cells
Blue histogram: HEK293 cells transduced to express a protein containing the EGFR tag
