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Human EMMPRIN/CD147 Antibody

Catalog #: AF972
9 Citations Datasheet / COA / SDS
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AF972
AF972-SP
Detection of Human EMMPRIN/CD147 by Western Blot.
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Product Details
Citations (9)
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Human EMMPRIN/CD147 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse EMMPRIN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
Thr25-His205
Accession # Q54A51
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB972).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human EMMPRIN/CD147 DuoSet ELISA Kit (Catalog # DY972) for convenient development of a sandwich ELISA or the Human EMMPRIN/CD147 Quantikine ELISA Kit (Catalog # DEMP00) for  a complete optimized ELISA.

 
Immunohistochemistry
5-15 µg/mL
See below
Knockout Validated
EMMPRIN/CD147 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in EMMPRIN/CD147 knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EMMPRIN/CD147 antibody by Western Blot. View Larger

Detection of Human EMMPRIN/CD147 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately 38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry EMMPRIN/CD147 antibody in Human Stomach Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

EMMPRIN/CD147 in Human Stomach Cancer Tissue. EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human EMMPRIN/ CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry EMMPRIN/CD147 antibody in Human Cervix by Immunohistochemistry (IHC-P). View Larger

EMMPRIN/CD147 in Human Cervix. EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human EMMPRIN/CD147 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human EMMPRIN/CD147 by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human EMMPRIN/CD147 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human EMMPRIN/CD147 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA View Larger

Human EMMPRIN/CD147 ELISA Standard Curve. Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMMPRIN/CD147

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44-66 kDa, variably glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily (1-4). Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) domain and a 41 aa intracellular domain. The ECD contains one C2-type and one V-type Ig-like domain. There is one 385 aa splice variant that contains an extra N-terminal IgCAM domain and is found only in the retina (5). mRNA transcripts, but not protein, have been reported for additional 432, 388, 205, 176, and 174 aa variants.

EMMPRIN is expressed in areas of tissue remodeling, including tumors, endometrium, placenta, skin, and regions undergoing angiogenesis (1, 2, 6-9). It is also expressed in cells with high metabolic activity, such as lymphoblasts, macrophages and tumor cells (2, 10). On cells with elevated metabolic rates, EMMPRIN is often co-expressed with the amino acid transporter CD98h (11). EMMPRIN also interacts with caveolin-1 (via its C2-like domain), and this reduces the level of EMMPRIN glycosylation and subsequent EMMPRIN multimerization and activity (12). EMMPRIN’s TM sequence contains a Glu and a Pro which are important for intracellular interactions with cyclophilins (CyP) (3, 13, 14). CyPA (cyclosporin A receptor) and CyP60 interactions with the TM segment promote leukocyte inflammatory chemotaxis and surface expression of EMMPRIN, respectively (13, 14). An active 22 kDa fragment can be shed from tumor cells by MT1-MMP (1). Tumor cells can also release active, full-length EMMPRIN in microvesicles (15, 16). Functionally, EMMPRIN is known to induce urokinase-type plasminogen activator (uPA), VEGF, hyaluronan, and multiple MMPs (1, 2, 7, 8, 9). Human EMMPRIN (269 aa) shows 58%, 58%, 62%, and 52% aa identity with mouse, rat, bovine, and chicken EMMPRIN, respectively. It also shows 25% and 38% aa identity with the related proteins, embigin and neuroplastin (SDR-1), respectively (4).

References
  1. Gabison, E.E. et al. (2005) Biochimie 87:361.
  2. Yurchenko, V. et al. (2006) Immunology 117:301.
  3. Kasinrerk, W. et al. (1992) J. Immunol. 149:847.
  4. Miyauchi, T. et al. (1991) J. Biochem. 110:770.
  5. Hanna, S.M. et al. (2003) BMC Biochem. 4:17.
  6. Riethdorf, S. et al. (2006) Int. J. Cancer 119:1800.
  7. Braundmeier, A.G. et al. (2006) J. Clin. Endocrinol. Metab. 91:2358.
  8. Tang, Y. et al. (2006) Mol. Cancer Res. 4:371.
  9. Quemener, C. et al. (2007) Cancer Res. 67:9.
  10. Wilson, M.C. et al. (2005) J. Biol. Chem. 280:27213.
  11. Xu, D. and M.E. Hemler (2005) Mol. Cell. Proteomics 4:1061.
  12. Tang, W. et al. (2004) Mol. Biol. Cell 15:4043.
  13. Arora, K. et al. (2005) J. Immunol. 175:517.
  14. Pushkarsky, T. et al. (2005) J. Biol. Chem. 280:27866.
  15. Egawa, N. et al. (2006) J. Biol. Chem. 281:37576.
  16. Sidhu, S.S. et al. (2004) Oncogene 23:956.
Long Name
Extracellular Matrix Metalloproteinase Inducer
Entrez Gene IDs
682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)
Alternate Names
5F7; basigin (Ok blood group); Basigin; BSG; CD147 antigen; CD147; Collagenase stimulatory factor; EMMPRIN; EMMPRINTCSF; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK blood group antigen; OK; Tumor cell-derived collagenase stimulatory factor

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Citations for Human EMMPRIN/CD147 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring
    Authors: N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu
    Nat Commun, 2020-09-07;11(1):4489.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  2. CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts
    Authors: M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima
    BMC Cancer, 2019-09-12;19(1):912.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. Efficacy Evaluation of Combination Treatment Using Gemcitabine and Radioimmunotherapy with 90Y-Labeled Fully Human Anti-CD147 Monoclonal Antibody 059-053 in a BxPC-3 Xenograft Mouse Model of Refractory Pancreatic Cancer
    Authors: A Sugyo, AB Tsuji, H Sudo, M Koizumi, Y Ukai, G Kurosawa, Y Kurosawa, T Saga, T Higashi
    Int J Mol Sci, 2018-09-29;19(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin
    Authors: D Gong, X Chi, K Ren, G Huang, G Zhou, N Yan, J Lei, Q Zhou
    Nat Commun, 2018-09-06;9(1):3623.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy
    Sci Rep, 2016-05-23;6(0):26463.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
    Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
    Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  7. Emmprin in epithelioid sarcoma: expression in tumor cell membrane and stimulation of MMP-2 production in tumor-associated fibroblasts.
    Authors: Koga K, Nabeshima K, Aoki M, Kawakami T, Hamasaki M, Toole BP, Nakayama J, Iwasaki H
    Int. J. Cancer, 2007-02-15;120(4):761-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Expression of emmprin and matrix metalloproteinases (MMPs) in peripheral nerve sheath tumors: emmprin and membrane-type (MT)1-MMP expressions are associated with malignant potential.
    Authors: Nabeshima K, Iwasaki H, Nishio J, Koga K, Shishime M, Kikuchi M
    Anticancer Res., 2006-03-01;26(2):1359-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A.
    Authors: Pushkarsky T, Zybarth G, Dubrovsky L, Yurchenko V, Tang H, Guo H, Toole B, Sherry B, Bukrinsky M
    Proc. Natl. Acad. Sci. U.S.A., 2001-05-15;98(11):6360-5.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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