Human EMR3 APC-conjugated Antibody

Catalog #: FAB8496A Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB8496A-025

Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry.

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Human EMR3 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Stains human EMR3 transfected but not irrelevant transfected cells in flow cytometry.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 908235

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human EMR3
Met1-Tyr652
Accession # NP_115960

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human EMR3 APC-conjugated Monoclonal Antibody (Catalog # FAB8496A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name

EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3

Entrez Gene IDs

84658 (Human)

Alternate Names

egf-like module containing, mucin-like, hormone receptor-like 3; EGF-like module receptor 3; EGF-like module-containing mucin-like hormone receptor-like 3; egf-like module-containing mucin-like receptor 3; EMR3