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Human Endoglin/CD105 Antibody

Catalog #: MAB10971
8 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10971
MAB10971-SP
Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Endoglin/CD105 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endoglin/CD105.
Source
Monoclonal Mouse IgG1 Clone # 166707
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105
Glu26-Gly586
Accession # Q5T9B9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Endoglin/CD105 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Endoglin/CD105 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Endoglin/CD105 Monoclonal Antibody (Catalog # MAB10971, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2‑5). Human Endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The human Endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine and canine Endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

References
  1. Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Human Endoglin/CD105 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The self-renewal dental pulp stem cell microtissues challenged by a toxic dental monomer
    Authors: G Kaufman, NM Kiburi, D Skrtic
    Biosci. Rep., 2020-06-26;40(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. CD105 (Endoglin) as negative prognostic factor in AML
    Authors: J Kauer, K Schwartz, C Tandler, C Hinterleit, M Roerden, G Jung, HR Salih, JS Heitmann, M Märklin
    Sci Rep, 2019-12-04;9(1):18337.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
    Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
    Mol Med Rep, 2019-02-01;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
    Stem Cells Int, 2016-10-20;2016(0):6104780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Human dermis harbors distinct mesenchymal stromal cell subsets.
    Authors: Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, Reinisch A, Strunk D, Elbe-Burger A
    J. Invest. Dermatol., 2011-11-03;132(3):563-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Validation of the cardiosphere method to culture cardiac progenitor cells from myocardial tissue.
    Authors: Davis D, Zhang Y, Smith R, Cheng K, Terrovitis J, Malliaras K, Li T, White A, Makkar R, Marban E
    PLoS ONE, 2009-09-25;4(9):e7195.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Characterization of human skin-derived mesenchymal stem cell proliferation rate in different growth conditions.
    Authors: Riekstina U, Muceniece R, Cakstina I, Muiznieks I, Ancans J
    Cytotechnology, 2009-02-14;58(3):153-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Therapeutic action of tranexamic acid in hereditary haemorrhagic telangiectasia (HHT): regulation of ALK-1/endoglin pathway in endothelial cells.
    Authors: Fernandez-L A, Garrido-Martin EM, Sanz-Rodriguez F, Ramirez JR, Morales-Angulo C, Zarrabeitia R, Perez-Molino A, Bernabeu C, Botella LM
    Thromb. Haemost., 2007-02-01;97(2):254-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human Endoglin/CD105 Antibody

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Human Endoglin/CD105 Antibody
By Abby Sukarto on 02/14/2020
Application: Flow Sample Tested: Bone Extracts Species: Human
Flow Cytometry Human Endoglin/CD105 Antibody MAB10971
Human Endoglin/CD105 Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human Endoglin/CD105 Antibody MAB10971
Human Endoglin/CD105 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human