Human Enolase 2/Neuron-specific Enolase Antibody
Human Enolase 2/Neuron-specific Enolase Antibody Summary
Met1-Leu434
Accession # P09104
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Enolase 2/Neuron‑specific Enolase in SH‑SY5Y Human Cell Line. Enolase 2/Neuron-specific Enolase was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Enolase 2/Neuron-specific Enolase Monoclonal Antibody (Catalog # MAB5169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Enolase 2/Neuron‑specific Enolase in Human Brain. Enolase 2/Neuron‑specific Enolase was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human Enolase 2/Neuron‑specific Enolase Monoclonal Antibody (Catalog # MAB5169) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Enolase 2/Neuron-specific Enolase
Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
Product Datasheets
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