Human eNOS Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human eNOS by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Immunoprecipitation of Human eNOS. eNOS was immunoprecipitated from lysates (1 x 106cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). Immunoprecipitated eNOS was detected by Western blot using 1 µg/mL Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950). View our recommended buffer recipes for immunoprecipitation.
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Detection of Human eNOS by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eNOS
Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.
Product Datasheets
Citations for Human eNOS Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy
Authors: Hussong, SA;Banh, AQ;Van Skike, CE;Dorigatti, AO;Hernandez, SF;Hart, MJ;Ferran, B;Makhlouf, H;Gaczynska, M;Osmulski, PA;McAllen, SA;Dineley, KT;Ungvari, Z;Perez, VI;Kayed, R;Galvan, V;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells
Authors: Z Wang, F Wang, X Kong, X Gao, Y Gu, J Zhang
Mediators Inflamm., 2019-06-17;2019(0):7162976.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot -
High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia
Authors: P Guerby, A Swiader, N Augé, O Parant, C Vayssière, K Uchida, R Salvayre, A Negre-Salv
Redox Biol, 2019-01-26;22(0):101126.
Species: Human
Sample Types: Cell Lysates, Tissue Homogenates
Applications: Immunoprecipitation -
Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??.
Authors: Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang
Molecular Medicine Reports, 2018-06-20;0(0):1791-3004.
Species: Human
Sample Types: Cell Lysate, Cell Lysates
Applications: Western Blot -
Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
IUBMB Life, 2018-03-15;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells.
Authors: Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T
Am. J. Physiol., Cell Physiol., 2009-12-30;298(3):C647-55.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Regional differences in prostaglandin E2 and nitric oxide production in the knee meniscus in response to dynamic compression.
Authors: Hennerbichler A, Fermor B, Hennerbichler D, Weinberg JB, Guilak F
Biochem. Biophys. Res. Commun., 2007-05-14;358(4):1047-53.
Species: Porcine
Sample Types: Cell Lysates
Applications: Western Blot -
Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Authors: Wright JL, Tai H, Churg A
Am. J. Respir. Cell Mol. Biol., 2004-07-08;31(5):501-9.
Species: Guinea Pig
Sample Types: Whole Tissue
Applications: IHC-P
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