Human ENPP-3/CD203c Antibody

Catalog #: MAB5756 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5756
MAB5756-SP

Detection of ENPP‑3/CD203c in Human IL‑3R alpha +PBMCs by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ENPP-3/CD203c Antibody Summary

Species Reactivity

Human

Specificity

Detects human ENPP‑3/CD203c.

Source

Monoclonal Mouse IgG₁ Clone # NP4D6

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human ENPP‑3/CD203c
Accession # O14638

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ENPP‑3/CD203c in Human IL‑3R alpha ⁺PBMCs by Flow Cytometry. Human IL-3Ra⁺peripheral blood mononuclear cells were stained with Mouse Anti-Human ENPP-3/CD203c Monoclonal Antibody (Catalog # MAB5756) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B) and Human IL-3 Ra Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB301P).Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ENPP-3/CD203c

CD203c is a 150 kDa type II transmembrane glycoprotein with a short cytoplasmic tail. CD203c is also known as ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP-3) and modulates purinergic signaling by hydrolysis of nucleotide triphosphates. CD203c is expressed on activated basophils and mast cells during allergic reactions, cardiac muscle, hepatocytes, and epithelial cells. It is upregulated in colon and bile duct carcinomas. Within the ECD, human CD203c shares 81% aa sequence identity with mouse and rat CD203.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 3

Entrez Gene IDs

5169 (Human)

Alternate Names

B10; CD203c antigen; CD203c; dJ1005H11.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); dJ914N13.3 (phosphodiesterase I/nucleotide pyrophosphatase 3); ectonucleotide pyrophosphatase/phosphodiesterase 3; ectonucleotide pyrophosphatase/phosphodiesterase family member 3; E-NPP 3; ENPP3; ENPP-3; gp130RB13-6; NPP3; PD-IBETA; PDNP3; PDNP3B10; Phosphodiesterase I beta; Phosphodiesterase I/nucleotide pyrophosphatase 3; phosphodiesterase-I beta