Human
EpCAM/TROP-1 Antibody

Catalog #: MAB9601
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9601-SP
MAB9601-100
MAB9601-500

Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry.

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All EpCAM/TROP1 Antibodies

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Citations (5)

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Human
EpCAM/TROP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human EpCAM/TROP-1 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 158206

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1
Extracellular domain

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human EpCAM/TROP-1 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human EpCAM/TROP‑1 Biotinylated Antibody (Catalog # BAF960)

Standard: Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 960-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human EpCAM/TROP-1 Monoclonal Antibody (Catalog # MAB9601, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca²⁺ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -Actinin (5).

References

Balzar, M. et al. (1999) J. Mol. Med. 77:699.
Boer, C.J, et al. (1999) J. Pathol. 188:201.
Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Long Name

Epithelial Cell Adhesion Molecule

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Alternate Names

17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

Product Datasheets

Product Datasheet

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Citations for Human
EpCAM/TROP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
PLoS ONE, 2019-05-02;14(5):e0215990.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection
Single-cell magnetic imaging using a quantum diamond microscope.
Authors: Glenn D, Lee K, Park H, Weissleder R, Yacoby A, Lukin M, Lee H, Walsworth R, Connolly C
Nat Methods, 2015-06-22;12(8):736-8.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development
Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
BMC Cancer, 2015-05-07;15(0):372.
Species: Human
Sample Types: BALF
Applications: ELISA Development (Capture)
Digital diffraction analysis enables low-cost molecular diagnostics on a smartphone.
Authors: Im H, Castro C, Shao H, Liong M, Song J, Pathania D, Fexon L, Min C, Avila-Wallace M, Zurkiya O, Rho J, Magaoay B, Tambouret R, Pivovarov M, Weissleder R, Lee H
Proc Natl Acad Sci U S A, 2015-04-13;112(18):5613-8.
Species: Human
Sample Types: Whole Cells
Applications: Immunoassay Development
Rapid detection and profiling of cancer cells in fine-needle aspirates.
Authors: Lee H, Yoon TJ, Figueiredo JL, Swirski FK, Weissleder R
Proc. Natl. Acad. Sci. U.S.A., 2009-07-20;106(30):12459-64.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry