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EpCAM/TROP-1 Antibody

Human
EpCAM/TROP-1 Antibody

Catalog #: AF960
13 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF960
AF960-SP
Detection of Human EpCAM/TROP‑1 by Western Blot.
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Product Details
Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human
EpCAM/TROP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain
Gln24-Lys265
Accession # CAA32870
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
10 µg/mL
A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL)
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
0.3-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EpCAM/TROP-1 antibody by Western Blot. View Larger

Detection of Human EpCAM/TROP‑1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of EpCAM/TROP-1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry EpCAM/TROP-1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

EpCAM/TROP‑1 in BG01V Human Embryonic Stem Cells. EpCAM/TROP-1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry EpCAM/TROP-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry EpCAM/TROP-1 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western View Larger

Detection of Human EpCAM/TROP‑1 by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for EpCAM/TROP‑1 at approximately 52 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry View Larger

Detection of EpCAM/TROP‑1 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and Jurkat Human Acute T Cell Leukemia Cell Line (Negative) Cells. EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Jurkat human acute T cell leukemia cell line (negative) cells using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamous epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

References
  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J. et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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Citations for Human
EpCAM/TROP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Generation and expansion of transitional lung organoids from human pluripotent stem cells
    Authors: IM Leko, N Schrode, J Torres, M Pezet, TA Thimraj, KG Beaumont, HW Snoeck
    bioRxiv : the preprint server for biology, 2023-02-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Prostate cancer extracellular vesicle digital scoring assay - a rapid noninvasive approach for quantification of disease-relevant mRNAs
    Authors: JJ Wang, N Sun, YT Lee, M Kim, T Vagner, K Rohena-Riv, Z Wang, Z Chen, RY Zhang, J Lee, C Zhang, H Tang, J Widjaja, TX Zhang, D Qi, PC Teng, YJ Jan, KC Hou, C Hamann, HM Sandler, TJ Daskivich, DJ Luthringer, NA Bhowmick, R Pei, S You, DD Vizio, HR Tseng, JF Chen, Y Zhu, EM Posadas
    Nano Today, 2023-01-03;48(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Capture Assay
  3. Targeted Inhibition of LPL/FABP4/CPT1 fatty acid metabolic axis can effectively prevent the progression of nonalcoholic steatohepatitis to liver cancer
    Authors: H Yang, Q Deng, T Ni, Y Liu, L Lu, H Dai, H Wang, W Yang
    International journal of biological sciences, 2021-10-11;17(15):4207-4222.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  4. Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids
    Authors: J Zhang, LTH Nguyen, R Hickey, N Walters, X Wang, KJ Kwak, LJ Lee, AF Palmer, E Reátegui
    Scientific Reports, 2021-04-13;11(1):8034.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  5. Improving hexaminolevulinate enabled cancer cell detection in liquid biopsy immunosensors
    Authors: KM Chan, J Gleadle, J Li, TD Michl, K Vasilev, M MacGregor
    Scientific Reports, 2021-03-31;11(1):7283.
    Species: Human
    Sample Types: Urine
    Applications: ELISA Capture
  6. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
    Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
    Cell Rep, 2020-10-13;33(2):108253.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  7. Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring
    Authors: N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu
    Nat Commun, 2020-09-07;11(1):4489.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  8. Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
    Authors: S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi
    Sci Rep, 2020-04-06;10(1):5989.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system
    Authors: D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen
    Sci Rep, 2018-05-29;8(1):8222.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration
    Sci Technol Adv Mater, 2016-11-25;17(1):807-815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  11. HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone
    Cancer Res., 2016-10-28;0(0):.
    Species: Human
    Sample Types: Whole Blood
    Applications: CTC
  12. EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells that Promote Epithelial-to-Mesenchymal Transition
    Cancer Res, 2016-08-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  13. Stromal gene expression defines poor-prognosis subtypes in colorectal cancer.
    Authors: Calon A, Lonardo E, Berenguer-Llergo A, Espinet E, Hernando-Momblona X, Iglesias M, Sevillano M, Palomo-Ponce S, Tauriello D, Byrom D, Cortina C, Morral C, Barcelo C, Tosi S, Riera A, Attolini C, Rossell D, Sancho E, Batlle E
    Nat Genet, 2015-02-23;47(4):320-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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EpCAM/TROP-1 Antibody

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