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Human ER alpha /NR3A1 Antibody

Catalog #: PP-H4624-00
2 Citations Datasheet / COA / SDS
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PP-H4624-00
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ER alpha /NR3A1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human ER alpha and cross-reacts with mouse and rat ER alpha. This antibody does not recognize human ER beta. Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H4624
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human ER alpha/NR3A1
aa 2-180
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human ER alpha.
 
Immunohistochemistry
This antibody can be used at 10 - 20 μg/mL withthe appropriate secondary reagents to detect human ER alpha.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human ER alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: ER alpha/NR3A1

Estrogen Receptor alpha (ER alpha, ER; NR3A1) is a member of the Steroid Receptor family. The natural ligand for ER is the classic estrogenic compound 17 beta -estradiol. ER alpha is expressed in a wide variety of tissues such as bones, uterus, bladder, ovary, prostate, testis, epididymis, kidney, breast, heart, vessel wall, pituitary and hypothalamus. ER has a variety of central physiological roles, including those involved in maintenance of the reproductive, cardiovascular, musculoskeletal and central nervous systems. ER alpha has been shown to form homodimers as well as heterodimers with ER beta. Both ER alpha and ER beta can give rise to numerous isoforms.

Long Name
Estrogen Receptor alpha
Entrez Gene IDs
2099 (Human)
Alternate Names
ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1

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Citations for Human ER alpha /NR3A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Progesterone and levonorgestrel regulate expression of 17betaHSD-enzymes in progesterone receptor positive breast cancer cell line T47D.
    Authors: Sivik T, Jansson A
    Biochem. Biophys. Res. Commun., 2012-04-30;422(1):109-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Increased NOS2 predicts poor survival in estrogen receptor-negative breast cancer patients.
    Authors: Glynn SA, Boersma BJ, Dorsey TH, Yi M, Yfantis HG, Ridnour LA, Martin DN, Switzer CH, Hudson RS, Wink DA, Lee DH, Stephens RM, Ambs S
    J. Clin. Invest., 2010-10-18;120(11):3843-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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