Human ErbB2/Her2 APC-conjugated Antibody Summary
Thr23-Thr652
Accession # P04626
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of ErbB2/Her2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB2/Her2 APC-conjugated Monoclonal Antibody (Catalog # FAB1129A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: ErbB2/Her2
ErbB2, also called Neu and Her2 (Human Epidermal Growth Factor Receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGF R), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-Kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-Catenin and Plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).
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Product Datasheets
Citations for Human ErbB2/Her2 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Avinash Test 7/27 447
Authors: Avinash
ACS Biomater Sci Eng. 2022 Jun 13;8(6):2307-2320
Species: Allium cepa
Sample Types:
Applications: Activity Assay -
Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer
Authors: Y Wang, L Guo, L Feng, W Zhang, T Xiao, X Di, G Chen, K Zhang
Oncol. Rep., 2018-03-20;39(5):2147-2159.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Asymmetric segregation of template DNA strands in basal-like human breast cancer cell lines.
Authors: Liu W, Jeganathan G, Amiri S, Morgan K, Ryan B, Pine S
Mol Cancer, 2013-11-15;12(1):139.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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