Human ErbB2/Her2 Herstatin Isoform Antibody Summary
Thr23-Gly419
Accession # AAD56009
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
ErbB2/Her2 in Human Breast. ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast using Mouse Anti-Human ErbB2/Her2 Herstatin Isoform Monoclonal Antibody (Catalog # MAB11291) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB2/Her2
Herstatin is a secreted 68 kDa glycoprotein encoded by an alternate transcript of the HER-2/neu (erbB-2) gene containing the N-terminal 304 amino acids (aa) of subdomain I and II, and 79 intron-encoded aa at the C-terminus. It is an endogenous inhibitor of the EGF receptor family that disrupts receptor interactions and inhibits growth of tumor cells that overexpress HER-2.
Product Datasheets
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma