Human ErbB3/Her3 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB3481N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3481N-100UG

Detection of ErbB3/Her3 in MCF‑7 Human Cell Line by Flow Cytometry.

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Human ErbB3/Her3 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ErbB3/Her3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human EGF R is observed.

Source

Monoclonal Mouse IgG₁ Clone # 66223

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ErbB3/Her3 in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3481N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB3/Her3

ErbB3, also known as Her3 (Human Epidermal Growth Factor Receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the Heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of Phosphoinositide 3-Kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

References

Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
Schlessinger, J. (2000) Cell 103:211.
Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Alternate Names

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)