Human ErbB3/Her3 Antibody Summary
Ser20-Thr643
Accession # P21860
Applications
Human ErbB3/Her3 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Cell Proliferation Induced by NRG1‑ beta 1/HRG1‑ beta 1 and Neutralization by Human ErbB3/Her3 Antibody. Recombinant Human NRG1-1 beta 1/HRG1-1 beta 1 (Catalog # 377-HB) stimulates proliferation in the MCF-17 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1-1 beta 1/HRG1-1 beta 1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human ErbB3/Her3 Monoclonal Antibody (Catalog # MAB3481). The ND50 is typically 0.0075-0.03 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB3/Her3
ErbB3, also called Her3 (human epidermal growth factor receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.
- Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
- Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
- Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
- Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
- Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
- Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
- Schlessinger, J. (2000) Cell 103:211.
- Daly, R.J. (1999) Growth Factors 16:255.
Product Datasheets
Citations for Human ErbB3/Her3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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DUSP6 inhibition overcomes neuregulin/HER3-driven therapy tolerance in HER2+ breast cancer
Authors: Momeny, M;Tienhaara, M;Sharma, M;Chakroborty, D;Varjus, R;Takala, I;Merisaari, J;Padzik, A;Vogt, A;Paatero, I;Elenius, K;Laajala, TD;Kurppa, KJ;Westermarck, J;
EMBO molecular medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adding of neurotensin to non-small cell lung cancer cells increases tyrosine phosphorylation of HER3
Authors: TW Moody, I Ramos-Alva, RT Jensen
Peptides, 2022-08-03;156(0):170858.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
Oncol Rep, 2020-10-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma
Authors: J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau
Sci Adv, 2020-02-26;6(9):eaax3223.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
A high-resolution study of in situ surface-enhanced Raman scattering nanotag behavior in biological systems
Authors: J Wang, W Anderson, J Li, LL Lin, Y Wang, M Trau
J Colloid Interface Sci, 2018-11-12;537(0):536-546.
Species: Human
Sample Types: Recombinant Protein
Applications: Dynamic Light Scattering -
Soluble HER3 predicts survival in bladder cancer patients
Authors: AA Memon, SC Gilliver, M Borre, J Sundquist, K Sundquist, E Nexo, BS Sorensen
Oncol Lett, 2017-11-22;15(2):1783-1788.
Species: Human
Sample Types: Plasma
Applications: ELISA Development (Capture) -
Neuregulin 1 allosterically enhances the anti-tumor effects of the non-competing anti-HER3 antibody 9F7-F11 by increasing its binding to HER3
Authors: C Le Clorenn, H Bazin, O Dubreuil, C Larbouret, C Ogier, Y Lazrek, V Garambois, MA Poul, P Mondon, JM Barret, G Mathis, JF Prost, A Pèlegrin, T Chardès
Mol. Cancer Ther., 2017-05-15;0(0):.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: ELISA Development, Neutralization -
Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs
Authors: Helmout Modjtahedi
Int. J. Oncol., 2016-09-05;49(5):1825-1838.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Neuregulin-1-mediated ErbB2-ErbB3 signalling protects human trophoblasts against apoptosis to preserve differentiation
Authors: V Fock, K Plessl, P Draxler, GR Otti, C Fiala, M Knöfler, J Pollheimer
J. Cell. Sci, 2015-10-21;128(23):4306-16.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Growth response of human colorectal tumour cell lines to treatment with afatinib (BIBW2992), an irreversible erbB family blocker, and its association with expression of HER family members.
Authors: Khelwatty SA, Essapen S, Seddon AM, Modjtahedi H
Int. J. Oncol., 2011-05-25;39(2):483-91.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
Authors: Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
Assay Drug Dev Technol, 2010-02-01;8(1):27-36.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Luminex Development -
Soluble ErbB3 levels in bone marrow and plasma of men with prostate cancer.
Authors: Lin SH, Lee YC, Choueiri MB, Wen S, Mathew P, Ye X, Do KA, Navone NM, Kim J, Tu SM, Yu-Lee LY, Logothetis CJ
Clin. Cancer Res., 2008-06-15;14(12):3729-36.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
A 45-kDa ErbB3 secreted by prostate cancer cells promotes bone formation.
Authors: Lin SH, Cheng CJ, Lee YC, Ye X, Tsai WW, Kim J, Pasqualini R, Arap W, Navone NM, Tu SM, Hu M, Yu-Lee LY, Logothetis CJ
Oncogene, 2008-05-19;27(39):5195-203.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
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