Human ErbB3/Her3 Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (5 ug), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL), Human Milk (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human ErbB3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human ErbB3/Her3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB3 in cell culture supernates, cell lysates, serum, plasma, saliva, urine and human milk. It contains NS0-expressed recombinant human ErbB3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB3 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ErbB3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean (pg/mL) |
396 |
1176 |
2446 |
437 |
1317 |
2646 |
| Standard Deviation |
4.56 |
11.2 |
36.9 |
29.4 |
66.1 |
119 |
| CV% |
1.2 |
1 |
1.5 |
6.7 |
5 |
4.5 |
Recovery
The recovery of human ErbB3 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
97 |
92-100 |
| Cell Lysis Buffer (n=2) |
98 |
96-101 |
| EDTA Plasma (n=4) |
97 |
93-103 |
| Heparin Plasma (n=4) |
96 |
91-102 |
| Human Milk (n=4) |
96 |
91-103 |
| Saliva (n=4) |
100 |
94-106 |
| Serum (n=4) |
100 |
93-109 |
| Urine (n=4) |
97 |
94-102 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB3 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Human ErbB3/Her3 ELISA Standard Curve
Product Datasheets
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Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ErbB3/Her3
ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB3 binds neuregulins NRG1 and NRG2, but lacks a working kinase domain; heterodimerization with another ErbB receptor is required for signaling. ErbB3 is important in development of Schwann cells, neural crest cells, heart valves and the sympathetic nervous system. When expressed with ErbB2 in breast, skin, ovary and gastrointestinal tumors, ErbB3 is a potent mitogen.
Long Name:
Receptor Tyrosine Protein Kinase ErbB3
Entrez Gene IDs:
2065 (Human); 13867 (Mouse)
Alternate Names:
c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
