Human ERK1 Antibody

Catalog #: AF1940 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1940
AF1940-SP

Detection of Human ERK1 by Western Blot.

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Human ERK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ERK1 in Western blots. Because the peptide immunogen corresponds to a region of ERK1 largely absent in ERK2, no reactivity with ERK2 is present.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Human ERK1 synthetic peptide
Met1-Val28
Accession # P27361

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ERK1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1940) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Rec-ombinant Human Active ERK1 (Catalog # 1879-KS) and Recom-binant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 42 kDa (as in-dicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1

ERK1 is a protein Serine/Threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain, and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors.

Long Name

Extracellular Signal-regulated Kinase 1

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Alternate Names

EC 2.7.11; ERK1; ERK-1; ERK1p44-MAPK; ERT2; Extracellular signal-regulated kinase 1; extracellular signal-related kinase 1; HS44KDAP; HUMKER1A; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK3; MGC20180; Microtubule-associated protein 2 kinase; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 3; P44ERK1; p44-ERK1; p44mapk; PRKM3; PRKM3EC 2.7.11.24