Human FABP2/I-FABP ELISA Kit - Quantikine

Catalog # Availability Size / Price Qty
DFBP20
Control Products Available
Best Seller
Human FABP2/I-FABP ELISA Linearity
1 Image
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (3)

Human FABP2/I-FABP Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
Sensitivity
6.21 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human FABP2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 77.5 207 425 80.5 216 446
Standard Deviation 3.14 7.24 12.4 8.94 17.4 26.6
CV% 4.1 3.5 2.9 11.1 8.1 6

Recovery

The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 96-104
Cell Lysis Buffer (n=2) 83 81-85
EDTA Plasma (n=4) 97 85-102
Heparin Plasma (n=4) 95 96-104
Serum (n=4) 101 97-106
Urine (n=4) 90 85-95

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FABP2/I-FABP ELISA Linearity

Product Datasheets

You must select a language.

x

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Long Name:
Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs:
2169 (Human); 25598 (Rat)
Alternate Names:
FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human FABP2/I-FABP Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. HIV-associated gut microbial alterations are dependent on host and geographic context
    Authors: Rocafort, M;Gootenberg, DB;Luévano, JM;Paer, JM;Hayward, MR;Bramante, JT;Ghebremichael, MS;Xu, J;Rogers, ZH;Munoz, AR;Okello, S;Kim, JH;Sentongo, R;Wagubi, R;Lankowski, A;Maruapula, S;Zhao, G;Handley, SA;Mosepele, M;Siedner, MJ;Kwon, DS;
    Nature communications
    Species: Human hepegivirus
    Sample Types: Plasma
  2. Enteric fungi protect against intestinal ischemia-reperfusion injury via inhibiting the SAA1-GSDMD pathway
    Authors: Chen, Y;Han, B;Guan, X;Du, G;Sheng, B;Tang, X;Zhang, Q;Xie, H;Jiang, X;Tan, Q;Chen, S;Wang, J;Chen, W;Xiao, W;
    Journal of advanced research
    Species: Mouse
    Sample Types: Feces
  3. The Clinical Significance of Serum Biomarkers of the Intestinal Barrier in Systemic Sclerosis: A Cross-Sectional Study
    Authors: A Stec, M Maciejewsk, M Zaremba, K Paralusz-S, M Michalska, L Rudnicka, M Sikora
    Journal of personalized medicine, 2023-04-18;13(4):.
    Species: Human
    Sample Types: Serum
  4. Effects of Flavonoid-Rich Orange Juice Intervention on Major Depressive Disorder in Young Adults: A Randomized Controlled Trial
    Authors: J Choi, JH Kim, M Park, HJ Lee
    Nutrients, 2022-12-28;15(1):.
    Species: Human
    Sample Types: Serum
  5. Reduced circulating FABP2 in patients with moderate to severe COVID-19 may indicate enterocyte functional change rather than cell death
    Authors: G Assante, A Tourna, R Carpani, F Ferrari, D Prati, F Peyvandi, F Blasi, A Bandera, A Le Guennec, S Chokshi, VC Patel, IJ Cox, L Valenti, NA Youngson
    Scientific Reports, 2022-11-05;12(1):18792.
    Species: Human
    Sample Types: Plasma
  6. Altered gut microbiota and its metabolites correlate with plasma cytokines in schizophrenia inpatients with aggression
    Authors: H Deng, L He, C Wang, T Zhang, H Guo, H Zhang, Y Song, B Chen
    BMC Psychiatry, 2022-09-27;22(1):629.
    Species: Human
    Sample Types: Serum
  7. Gut biomolecules (I-FABP, TFF3 and lipocalin-2) are associated with linear growth and biomarkers of environmental enteric dysfunction (EED) in Bangladeshi children
    Authors: MM Hasan, MA Gazi, S Das, SM Fahim, F Hossaini, AR Khan, J Ferdous, MA Alam, M Mahfuz, T Ahmed
    Scientific Reports, 2022-08-16;12(1):13905.
    Species: Human
    Sample Types: Plasma
  8. Plasma Microbiome in COVID-19 Subjects: An Indicator of Gut Barrier Defects and Dysbiosis
    Authors: R Prasad, MJ Patton, JL Floyd, S Fortmann, M DuPont, A Harbour, J Wright, R Lamendella, BR Stevens, GY Oudit, MB Grant
    International Journal of Molecular Sciences, 2022-08-15;23(16):.
    Species: Human
    Sample Types: Plasma
  9. Intestinal fatty acid binding protein (I-FABP) and CXC3L1 evaluation as biomarkers for patients at high-risk for coeliac disease in Johannesburg, South Africa
    Authors: A Gandini, T De Maayer, C Munien, K Bertrand, R Cairns, A Mayne, MP Gededzha, ES Mayne
    Cytokine, 2022-07-13;157(0):155945.
    Species: Human
    Sample Types: Serum
  10. Intestinal fatty acid binding protein is a disease biomarker in paediatric coeliac disease and Crohn's disease
    Authors: M Logan, M MacKinder, CM Clark, A Kountouri, M Jere, UZ Ijaz, R Hansen, P McGrogan, RK Russell, K Gerasimidi
    BMC gastroenterology, 2022-05-23;22(1):260.
    Species: Human
    Sample Types: Plasma
  11. Translocating lipopolysaccharide correlates with the severity of enterovirus A71-induced HFMD by promoting pro-inflammation and viral IRES activity
    Authors: Y Wang, K Dan, X Xue, X Yang, X Feng, Q Yang, J Yang, B Chen
    Gut pathogens, 2021-11-22;13(1):69.
    Species: Human
    Sample Types: Serum
  12. Impact of 12-week exercise program on biomarkers of gut barrier integrity in patients with coronary artery disease
    Authors: V Feng, KK Bawa, S Marzolini, A Kiss, P Oh, N Herrmann, KL Lanctôt, D Gallagher
    PLoS ONE, 2021-11-19;16(11):e0260165.
    Species: Human
    Sample Types: Serum
  13. Allergy-related diseases and early gut fungal and bacterial microbiota abundances in children
    Authors: K Schei, MR Simpson, T Øien, S Salamati, K Rudi, RA Ødegård
    Clinical and translational allergy, 2021-06-28;11(5):e12041.
    Species: Human
    Sample Types: Serum
  14. Identifying a biological signature of prenatal maternal stress
    Authors: JM Keane, AS Khashan, FP McCarthy, LC Kenny, JM Collins, SM O'Donovan, JR Brown, JF Cryan, TG Dinan, G Clarke, SM O'Mahony
    JCI Insight, 2021-01-25;0(0):.
    Species: Human
    Sample Types: Serum
  15. Long-lasting severe immune dysfunction in Ebola virus disease survivors
    Authors: A Wiedemann, E Foucat, H Hocini, C Lefebvre, BP Hejblum, M Durand, M Krüger, AK Keita, A Ayouba, S Mély, JC Fernandez, A Touré, S Fourati, C Lévy-March, H Raoul, E Delaporte, L Koivogui, R Thiébaut, C Lacabaratz, Y Lévy
    Nat Commun, 2020-07-24;11(1):3730.
    Species: Human
    Sample Types: Serum
  16. Epinephrine treatment but not time to ROSC is associated with intestinal injury in patients with cardiac arrest
    Authors: KA Krychtiuk, B Richter, M Lenz, P Hohensinne, K Huber, C Hengstenbe, J Wojta, G Heinz, WS Speidl
    Resuscitation, 2020-06-06;0(0):.
    Species: Human
    Sample Types: Serum
  17. Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
    Authors: LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs
    Mucosal Immunol, 2020-03-09;0(0):.
    Species: Human
    Sample Types:
  18. Gut permeability, inflammation, and bone density across the menopause transition
    Authors: A Shieh, M Epeldegui, AS Karlamangl, GA Greendale
    JCI Insight, 2020-01-30;0(0):.
    Species: Human
    Sample Types: Plasma
  19. Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
    Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
    Sci Rep, 2019-02-19;9(1):2304.
    Species: Human
    Sample Types: Plasma
  20. Relation between postoperative ileus and anastomotic leakage after colorectal resection: a post hoc analysis of a prospective randomized controlled trial
    Authors: E G Peters
    Colorectal Dis, 2017-07-01;0(0):.
    Species: Human
    Sample Types: Plasma

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

View all ELISA FAQs
Loading...

Reviews for Human FABP2/I-FABP Quantikine ELISA Kit

Average Rating: 4.7 (Based on 3 Reviews)

5 Star
66.67%
4 Star
33.33%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human FABP2/I-FABP Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human FABP2/I-FABP Quantikine ELISA Kit
By Adriana Trajtman on 10/20/2017
Sample Tested: blood and respiratory

Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 09/07/2017
Sample Tested: Serum

The Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.


Human FABP2/I-FABP Quantikine ELISA Kit
By Anonymous on 02/08/2017
Sample Tested: Human Plasma