Human FABP3/H-FABP Biotinylated Antibody

Catalog #: BAF1678 Datasheet / COA / SDS

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BAF1678

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Human FABP3/H-FABP Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human FABP3 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse (rm) FABP4 is observed, 20% cross-reactivity with recombinant rat (rr) FABP1 is observed, and 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human FABP3
Met1-Ala133
Accession # P05413

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human FABP3

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human heart

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FABP3/H‑FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human FABP3/H-FABP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP3/H-FABP

Human FABP3, also known as FABP-Heart (H-FABP), FABP-Muscle (M-FABP) and mammary derived growth inhibitor (MDGI), is a member of the intracellular FABP family. It plays a role in the transport of long chain fatty acids in the cytoplasm.

Long Name

Fatty Acid-Binding Protein 3

Entrez Gene IDs

2170 (Human)

Alternate Names

FABP11; FABP3; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart (mammary-derived growthinhibitor); Fatty acid-binding protein 3; Fatty acid-binding protein 3, muscle; fatty acid-binding protein, heart; Heart-type fatty acid-binding protein; HFABP; H-FABP; H-FABPM-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; O-FABP