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Human FABP4 ELISA Kit - Quantikine

Catalog #: DFBP40
14 Citations Datasheet / COA / SDS
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DFBP40
Human FABP4/A-FABP ELISA Standard Curve
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Citations (14)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human FABP4 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
14.2 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human FABP4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human FABP4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FABP4 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human FABP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FABP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FABP4.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 349 1155 2595 407 1236 2619
Standard Deviation 20.2 39.7 117 51.6 156 291
CV% 5.8 3.4 4.5 12.7 12.6 11.1

Recovery

The recovery of human FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 108 100-118
Cell Lysis Buffer 1 (n=2) 108 92-117
EDTA Plasma (n=4) 98 88-113
Heparin Plasma (n=4) 95 83-103
Serum (n=4) 99 82-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FABP4/A-FABP ELISA Linearity

Scientific Data

N/A Human FABP4/A-FABP ELISA Standard Curve View Larger

Human FABP4/A-FABP ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP4/A-FABP

FABP4 (Fatty Acid Binding Protein 4), also known as A-FABP and aP2, is the predominant FABP found in adipocytes and is often used as a marker for adipocyte differentiation. It is also expressed in macrophages, dendritic cells, and endothelial cells. FABP4 is a key mediator of intracellular fatty acid transport and metabolism in adipose tissue. Its expression is regulated by multiple factors including fatty acids, PPAR gamma agonists, and Insulin, and its levels increase with lipolytic stimulation. It can increase the hydrolytic activity of Hormone-Sensitive Lipase and increase the production of proinflammatory cytokines and chemokines. FABP4 upregulation in adipocytes and macrophages is associated with the development of Insulin resistance, hypertriacylglycerolaemia, and atherosclerosis. Serum levels of FABP4 are elevated in obesity and metabolic syndrome, type 2 diabetes, HIV-associated lipodystrophy, polycystic ovary syndrome, nonalcoholic fatty liver disease, atherosclerosis, and acute ischaemic stroke. FABP4 is also overexpressed in multiple cancer types including ovarian, bladder, glioblastoma, and oral, and it may play a role in tumor progression.

Long Name:
Fatty Acid-Binding Protein 4
Entrez Gene IDs:
2167 (Human); 11770 (Mouse); 79451 (Rat)
Alternate Names:
Adipocyte lipid-binding protein; Adipocyte-type fatty acid-binding protein; AFABP; A-FABP; A-FABPAFABP; ALBP; aP2; FABP4; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; fatty acid-binding protein, adipocyte
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human FABP4 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. The tyrosine kinase inhibitor Dasatinib reduces cardiac steatosis and fibrosis in obese, type 2 diabetic mice
    Authors: Gu, Y;Avolio, E;Alvino, VV;Thomas, AC;Herman, A;Miller, PJ;Sullivan, N;Faulkner, A;Madeddu, P;
    Cardiovascular diabetology
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  2. Inflammatory and metabolic markers in relation to outcome of in vitro fertilization in a cohort of predominantly overweight and obese women
    Authors: H Svensson, S Einarsson, D Olausson, L Kluge, C Bergh, S Edén, M Lönn, A Thurin-Kje
    Scientific Reports, 2022-08-03;12(1):13331.
    Species: Human
    Sample Types: Serum
  3. PET/MRI-Evaluated Activation of Brown Adipose Tissue via Cold Exposure Impacts Lipid Metabolism
    Authors: K Miniewska, K Maliszewsk, K Pietrowska, J Godzie?, ? ?abieniec, M Mojsak, A Kr?towski, M Ciborowski
    Metabolites, 2022-05-19;12(5):.
    Species: Human
    Sample Types: Serum
  4. The adipokine FABP4 is a key regulator of neonatal glucose homeostasis
    Authors: I Ron, RK Lerner, M Rathaus, R Livne, S Ron, E Barhod, R Hemi, A Tirosh, T Strauss, K Ofir, I Goldstein, IM Pessach, A Tirosh
    JCI Insight, 2021-10-22;6(20):.
    Species: Human
    Sample Types: Serum
  5. Serum fatty acid-binding protein 4 levels and responses of pancreatic islet &beta-cells and &alpha-cells in patients with type 2 diabetes
    Authors: H Wang, J Cao, JB Su, XQ Wang, X Wang, DM Zhang, XH Wang
    Diabetology & metabolic syndrome, 2021-06-26;13(1):70.
    Species: Human
    Sample Types: Serum
  6. Determination of FABP4, RBP4 and the MMP-9/NGAL complex in the serum of women with breast cancer
    Authors: D Tsakogiann, E Kalogera, F Zagouri, E Zografos, D Balalis, G Bletsa
    Oncology Letters, 2020-12-04;21(2):85.
    Species: Human
    Sample Types: Serum
  7. Monounsaturated fat rapidly induces hepatic gluconeogenesis and whole-body insulin resistance
    Authors: T Sarabhai, S Kahl, J Szendroedi, DF Markgraf, OP Zaharia, C Barosa, C Herder, F Wickrath, P Bobrov, JH Hwang, JG Jones, M Roden
    JCI Insight, 2020-05-21;5(10):.
    Species: Human
    Sample Types: Serum
  8. Plasma Adipokines in Patients Resuscitated from Cardiac Arrest: Difference of Visfatin between Survivors and Nonsurvivors
    Authors: YZ Chen, SQ Zhou, YQ Chen, H Peng, YG Zhuang
    Dis. Markers, 2020-01-14;2020(0):9608276.
    Species: Human
    Sample Types: Plasma
  9. Comparison of plasma fatty acid binding protein 4 concentration in venous and capillary blood
    Authors: S Numao, Y Nagasawa, N Goromaru, S Tamaki
    PLoS ONE, 2019-12-11;14(12):e0226374.
    Species: Human
    Sample Types: Plasma
  10. FABP4 in Gestational Diabetes-Association between Mothers and Offspring
    Authors: J Patro-Ma?y, M Trojnar, ? Kimber-Tro, R Mierzy?ski, J Bartosiewi, J Oleszczuk, B Leszczy?sk
    J Clin Med, 2019-02-27;8(3):.
    Species: Human
    Sample Types: Serum
  11. Development of Versatile Human In Vitro Vascularized Adipose Tissue Model with Serum-Free Angiogenesis and Natural Adipogenesis Induction
    Authors: O Huttala, M Palmroth, P Hemminki, T Toimela, T Heinonen, T Ylikomi, JR Sarkanen
    Basic Clin. Pharmacol. Toxicol., 2018-04-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Partitioning of adipose lipid metabolism by altered expression and function of PPAR isoforms after bariatric surgery
    Authors: C Jahansouz, H Xu, AV Hertzel, S Kizy, KA Steen, R Foncea, FJ Serrot, N Kvalheim, G Luthra, K Ewing, DB Leslie, S Ikramuddin, DA Bernlohr
    Int J Obes (Lond), 2017-08-14;42(2):139-146.
    Species: Human
    Sample Types: Serum
  13. Adipocyte Fatty-Acid Binding Protein is Overexpressed in Cirrhosis and Correlates with Clinical Outcomes
    Authors: I Graupera, M Coll, E Pose, C Elia, S Piano, E Solà, D Blaya, P Huelin, C Solé, R Moreira, G de Prada, N Fabrellas, A Juanola, M Morales-Ru, P Sancho-Bru, C Villanueva, P Ginès
    Sci Rep, 2017-05-12;7(1):1829.
    Species: Human
    Sample Types: Plasma
  14. Using Neonatal Skin to Study the Developmental Programming of Aging
    Authors: Leryn J Reynolds
    Exp. Gerontol, 2016-12-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates

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