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Human FATP1 PE-conjugated Antibody

Catalog #: IC3304P
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC3304P
Detection of FATP1 in HT‑29 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FATP1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human FATP1 in cell transfectants but not non-transfected parent cells in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 308420
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human FATP1
Met1-Leu646
Accession # Q6PCB7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of FATP1 antibody in HT-29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of FATP1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human FATP1 PE-conjugated Monoclonal Antibody (Catalog # IC3304P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: FATP1

FATP1 (Fatty Acid Transport Protein 1), also known as SLC27A1, is a 63-64 kDa protein that belongs to the ATP-dependent AMP-binding enzyme family. A type I transmembrane protein, FATP1 is one of six mammalian FATPs that catalyze an ATP-dependent esterification of long-chain fatty acids. FATP1 is expressed in cells with high rates of fatty acid uptake for storage of energy, principally in brown and white adipocytes, plus skeletal muscle myocytes. FATP1 likely functions as a homodimer, and is found in multiple locations such as cytoplasm, in intracellular membranes, and in the plasma membrane in response to insulin (in adipocytes). Full length FATP1 (amino acids 1-646) shares 89% amino acid sequence identity with mouse FATP1.

Long Name
Fatty Acid Transport Protein 1
Entrez Gene IDs
376497 (Human); 26457 (Mouse); 94172 (Rat)
Alternate Names
ACSVL5FATP-1; EC 6.2.1; EC 6.2.1.-; FATP1; FATP1Solute carrier family 27 member 1; FATPlong-chain fatty acid transport protein 1; Fatty acid transport protein 1; FLJ00336; MGC71751; SLC27A1; solute carrier family 27 (fatty acid transporter), member 1

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Citations for Human FATP1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Hydrogen Peroxide-Induced DNA Damage and Repair through the Differentiation of Human Adipose-Derived Mesenchymal Stem Cells
    Authors: M Valverde, J Lozano-Sal, P Fortini, MA Rodriguez-, E Rojas, E Dogliotti
    Stem Cells Int, 2018-10-10;2018(0):1615497.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Senescence Alters PPARG (Peroxisome Proliferator-Activated Receptor Gamma)-Dependent Fatty Acid Handling in Human Adipose Tissue Microvascular Endothelial Cells and Favors Inflammation
    Authors: A Briot, P Decaunes, F Volat, C Belles, M Coupaye, S Ledoux, A Bouloumié
    Arterioscler. Thromb. Vasc. Biol., 2018-03-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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