Human Fatty Acid Synthase/FASN Antibody

Catalog # Availability Size / Price Qty
AF5927
AF5927-SP
Detection of Human Fatty Acid Synthase/FASN by Western Blot.
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Human Fatty Acid Synthase/FASN Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fatty Acid Synthase/FASN in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Fatty Acid Synthase/FASN
Ser10-Cys212
Accession # P49327
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Knockout Validated
Fatty Acid Synthase/FASN is specifically detected in HEK293 human embryonic kidney parental cell line but is not detectable in Fatty Acid Synthase/FASN knockout HEK293 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Fatty Acid Synthase/FASN antibody by Western Blot. View Larger

Detection of Human Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Knockout Validated Western Blot Shows Human Fatty Acid Synthase/FASN Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293 human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockout HEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fatty Acid Synthase/FASN

FASN (Fatty Acid SyNthase; also FAS) is a cytosolic member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length. It contains multiple domains, including a beta ‑ketoacyl synthase domain (aa 1‑414), an acyl and malonyl transferase domain (aa 429‑817), an enoyl reductase domain (aa 1563‑1863), a beta ‑ketoacyl reductase domain (aa 1864‑2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 10‑212, human FASN shares 92% aa identity with mouse FASN.

Entrez Gene IDs
2194 (Human); 14104 (Mouse)
Alternate Names
EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; Fatty Acid Synthase; MGC14367; MGC15706; OA-519; SDR27X1

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