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Human Fc gamma  RII/CD32 Antibody

Catalog #: MAB1330
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1330-SP
MAB1330-500
MAB1330-100
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fc gamma  RII/CD32 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIA and human Fc gamma RIIB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Fc gamma  RII.
Source
Monoclonal Mouse IgG2A Clone # 190723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB
Ala46-Pro217
Accession # P31994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Fc gamma RIIA/CD32a (Catalog # 1330-CD)
Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes and granulocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RII/CD32

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T cells, and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name
Fc gamma Receptor II
Entrez Gene IDs
2212 (Human); 14130 (Mouse)
Alternate Names
CD32 antigen; CD32B; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RII; Fc gamma RIIb; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FcgRII; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b

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Citations for Human Fc gamma  RII/CD32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Activatory and inhibitory Fcgamma receptors augment rituximab-mediated internalization of CD20 independent of signaling via the cytoplasmic domain.
    Authors: Vaughan A, Chan C, Klein C, Glennie M, Beers S, Cragg M
    J Biol Chem, 2015-01-07;290(9):5424-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Alpha-1 Antitrypsin regulates human neutrophil chemotaxis induced by soluble immune complexes and IL-8.
    Authors: Bergin DA, Reeves EP, Meleady P, Henry M, McElvaney OJ, Carroll TP, Condron C, Chotirmall SH, Clynes M, O'Neill SJ, McElvaney NG
    J. Clin. Invest., 2010-11-08;120(12):4236-50.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  3. Myosin VI is required for sorting of AP-1B-dependent cargo to the basolateral domain in polarized MDCK cells.
    Authors: Au JS, Puri C, Ihrke G, Kendrick-Jones J, Buss F
    J. Cell Biol., 2007-04-02;177(1):103-14.
    Species: Canine
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human Fc gamma  RII/CD32 Antibody

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Human Fc gamma  RII/CD32 Antibody
By Anonymous on 05/03/2017
Application: IHC Sample Tested: Adult lung Species: Human
Immunohistochemistry Human Fc gamma  RII/CD32 Antibody MAB1330