Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody

Catalog #: BAM1875 Datasheet / COA / SDS

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BAM1875

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Human Fc gamma RIIB/C (CD32b/c) Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fc gamma RIIB/C in ELISAs. In ELISAs, this antibody shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.

Source

Monoclonal Mouse IgG_2A Clone # 190703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
Ala46-Pro217
Accession # P31994

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Human Fc gamma RIIB/CD32b Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human Fc gamma RIIB/C (CD32b/c) Antibody (Catalog # MAB18751)

Standard: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF (Catalog # 1875-CD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/C (CD32b/c)

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor IIB/C

Entrez Gene IDs

2213 (Human); 14130 (Mouse)

Alternate Names

CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b; Fc gamma RIIB/C (CD32b/c)