Human FCRL3/FcRH3 Antibody

Catalog #: AF3126 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3126
AF3126-SP

Detection of FCRL3/FcRH3 in Human Blood Lymphocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRL3/FcRH3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human FCRL3/FcRH3 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IRTA2 and rhIRTA5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human FCRL3/FcRH3
Arg14-Arg569
Accession # NP_443171

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of FCRL3/FcRH3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human FCRL3/FcRH3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3126) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108) and Mouse Anti-Human NKp46/NCR1 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB1850P). Quadrant markers were set based on control antibody staining (Catalog # AB-108-C).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL3/FcRH3

FCRL3 (Fc Receptor-Like 3), also known as FcRH3, IRTA3, and SPAP2, is a 110 kDa molecule with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1). Mature human FCRL3 consists of a 556 amino acid (aa) extracellular domain (ECD) with six Ig-like domains, a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain with four immunotyrosine inhibitory motifs (ITIMs) (2 - 4). Within the ECD, human and mouse FCRL3 share 35% aa sequence identity. Alternate splicing generates several additional isoforms with deletions or substitutions in both the extracellular and intracellular regions. These include potentially secreted forms that are truncated following the second Ig-like domain (4). FCRL3 is expressed in secondary lymphoid organs on the surface of mature naïve and memory B cells, NK cells, and B cell lines derived from chronic lymphocytic leukemias (2, 3, 5). It is upregulated on B cells following LPS or anti-CD40 stimulation (6). A polymorphism in the FCRL3 promoter induces enhanced transcription and is associated with the development of autoimmune disorders in a Japanese population (6, 7). Tyrosine phosphorylation within the ITIMs of FCRL3 enables its association with SHP-1 (4).

References

Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
Miller, I. et al. (2002) Blood, 99:2662.
Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. 98:9772.
Xu, M.-J. et al. (2002) Biochem. Biophys. Res. Commun. 293:1037.
Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
Kochi, Y. et al. (2005) Nat. Genet. 37:478.
Chistiakov, D.A. and A.P. Chistiakov (2007) Hum. Immunol. 68:375.

Long Name

Fc Receptor-like 3

Entrez Gene IDs

115352 (Human); 329693 (Mouse); 680697 (Rat)

Alternate Names

CD307c; Fc receptor homolog 3; Fc receptor-like 3; Fc receptor-like protein 3; FcRH3; FCRL3; fcR-like protein 3; hIFGP3; IFGP3; immunoglobulin superfamily receptor translocation associated protein 3; IRTA3; SPAP2