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Human FGF-23 Antibody

Catalog #: AF2604
5 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2604
AF2604-SP
Cell Proliferation Induced by FGF‑23 and Neutralization by Human FGF‑23 Antibody.
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Citations (5)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FGF-23 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-23 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human FGF-23
Tyr25-Ile251
Accession # Q9GZV9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.24 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FGF‑23 (Catalog # 2604-FG)
Neutralization
Measured by its ability to neutralize FGF‑23-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human FGF‑23, 5 µg/mL Recombinant Mouse Klotho, and 10 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF‑23 and Neutralization by Human FGF‑23 Antibody. View Larger

Cell Proliferation Induced by FGF‑23 and Neutralization by Human FGF‑23 Antibody. In the presence of Recombinant Mouse Klotho (5 µg/mL, Catalog # 1819-KL) and heparin (10 µg/mL), Recombinant Human FGF-23 (Catalog # 2604-FG) stimulates proliferation in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2604). The ND50 is typically 5-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF family, within a subfamily that also includes FGF-19 and FGF-21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). FGF-19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma-stabilizing intramolecular folds (2-4). Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134‑162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance (4-7). O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity (7, 8). Mature human FGF-23 shows 72% aa identity to mouse FGF-23 and is active on mouse cells (6). FGF-23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF-23 activity (9-11). FGF-23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels (12). FGF-23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by downregulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate (13).

References
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
  3. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  4. Harmer, N.J. et al. (2004) Biochemistry 43:629.
  5. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
  6. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
  7. Frishberg, Y. et al. (2007) J. Bone Miner. Res. 22:235.
  8. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
  9. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  10. Urakawa, I. et al. (2006) Nature 444:770.
  11. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  12. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
  13. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
 
Long Name
Fibroblast Growth Factor 23
Entrez Gene IDs
8074 (Human); 64654 (Mouse)
Alternate Names
ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor

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Citations for Human FGF-23 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Soluble alpha -klotho and heparin modulate the pathologic cardiac actions of fibroblast growth factor 23 in chronic kidney disease
    Authors: Christopher Yanucil, Dominik Kentrup, Isaac Campos, Brian Czaya, Kylie Heitman, David Westbrook et al.
    Kidney International
  2. Transcriptional profiling reveals roles of intercellular Fgf9 signaling in astrocyte maturation and synaptic refinement during brainstem development
    Authors: AN Brandebura, DR Kolson, EM Amick, J Ramadan, MC Kersting, RH Nichol, PS Holcomb, PH Mathers, P Stoilov, GA Spirou
    The Journal of Biological Chemistry, 2022-06-23;0(0):102176.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts
    Authors: ER Smith, SJ Tan, SG Holt, TD Hewitson
    Sci Rep, 2017-06-13;7(1):3345.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. FGF23 is endogenously phosphorylated in bone cells.
    Authors: Lindberg I, Pang H, Stains J, Clark D, Yang A, Bonewald L, Li K
    J Bone Miner Res, 2015-03-01;30(3):449-54.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  5. FGF23 neutralization improves chronic kidney disease-associated hyperparathyroidism yet increases mortality.
    Authors: Shalhoub V, Shatzen EM, Ward SC
    J. Clin. Invest., 2012-06-25;122(7):2543-53.
    Species: Mouse, Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Human FGF-23 Antibody
By Anonymous on 01/08/2018
Application: ELISA Sample Tested: Purified Standard Species: Human