Human Ficolin-2 Biotinylated Antibody Summary
Leu26-Ala313
Accession # Q15485
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ficolin-2
Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1‑4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35‑40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N‑terminal collagen domain and a C‑terminal fibrinogen‑like domain that includes a calcium binding site and two potential N‑glycosylation sites. The collagen domain mediates trimer formation. Larger homo‑multimers are formed by disulfide links at the N‑terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin‑2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N‑acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin‑2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin‑2. Some affect serum concentration, while others can increase or decrease ligand binding (11).
- Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.
- Endo, Y. et al. (1996) Genomics 36:515.
- Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.
- Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.
- Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.
- Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.
- Garlatti, V. et al. (2007) EMBO J. 26:623.
- Lynch, N. J. et al. (2004) J. Immunol. 172:1198.
- Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.
- Kuraya, M. et al. (2005) Immunobiology 209:689.
- Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.
Product Datasheets
Citation for Human Ficolin-2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Synergy between ficolin-2 and pentraxin 3 boosts innate immune recognition and complement deposition.
Authors: Ma YJ, Doni A, Hummelshoj T, Honore C, Bastone A, Mantovani A, Thielens NM, Garred P
J. Biol. Chem., 2009-07-24;284(41):28263-75.
Species: Human
Sample Types: Serum
Applications: Western Blot
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