Human FoxF1 Antibody Summary
Met154-Met379
Accession # Q12946
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FoxF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human FoxF1 by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse FoxF1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxF1
FoxF1 belongs to a large family of proteins that share a common forkhead/winged helix DNA binding domain. FoxF1 is implicated in pulmonary morphogenesis, specifically in development of the lung mesenchyme. Experiments in mice indicate that haploinsufficiency of FoxF1 can lead to perinatal lethality due to pulmonary abnormalities. In addition expression of FoxF1 can be induced by hedgehog ligands and appears to regulate expression of BMP-4.
Product Datasheets
Citations for Human FoxF1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling
Authors: Bian, F;Lan, YW;Zhao, S;Deng, Z;Shukla, S;Acharya, A;Donovan, J;Le, T;Milewski, D;Bacchetta, M;Hozain, AE;Tipograf, Y;Chen, YW;Xu, Y;Shi, D;Kalinichenko, VV;Kalin, TV;
Nature communications
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells
Authors: AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells
Cell Stem Cell, 2021-12-01;0(0):.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC -
The embryonic ontogeny of the gonadal somatic cells in mice and monkeys
Authors: K Sasaki, A Oguchi, K Cheng, Y Murakawa, I Okamoto, H Ohta, Y Yabuta, C Iwatani, H Tsuchiya, T Yamamoto, Y Seita, M Saitou
Cell Reports, 2021-05-04;35(5):109075.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
FOXF1 is required for the oncogenic properties of PAX3-FOXO1 in rhabdomyosarcoma
Authors: D Milewski, S Shukla, BE Gryder, A Pradhan, J Donovan, P Sudha, S Vallabh, A Pyros, Y Xu, A Barski, S Szabo, B Turpin, JG Pressey, DP Millay, J Khan, VV Kalinichen, TV Kalin
Oncogene, 2021-02-24;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4
Authors: DG Belair, G Lu, LE Waller, JA Gustin, ND Collins, KL Kolaja
Sci Rep, 2020-02-18;10(1):2864.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Hedgehog signaling patterns the oral-aboral axis of the mandibular arch
Authors: J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang
Elife, 2019-01-14;8(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
FOXF1 Inhibits Pulmonary Fibrosis by Preventing CDH2-CDH11 Cadherin Switch in Myofibroblasts
Authors: M Black, D Milewski, T Le, X Ren, Y Xu, VV Kalinichen, TV Kalin
Cell Rep, 2018-04-10;23(2):442-458.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP -
Wnt/?-catenin promotes gastric fundus specification in mice and humans
Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
Nature, 2017-01-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
FoxF1 and FoxF2 transcription factors synergistically promote rhabdomyosarcoma carcinogenesis by repressing transcription of p21(Cip1) CDK inhibitor
Oncogene, 2016-07-18;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A Molecular atlas of Xenopus respiratory system development.
Authors: Rankin S, Thi Tran H, Wlizla M, Mancini P, Shifley E, Bloor S, Han L, Vleminckx K, Wert S, Zorn A
Dev Dyn, 2014-09-11;244(1):69-85.
Species: Xenopus
Sample Types: Whole Tissue
Applications: IHC-P
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