Human GADD45 alpha Antibody

Catalog #: AF5510 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5510
AF5510-SP

Detection of Human GADD45 alpha by Western Blot.

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Human GADD45 alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human GADD45 alpha in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GADD45 alpha
Met1-Arg165
Accession # P24522

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human GADD45 alpha by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line either mock transfected or transfected with full length human GADD45a. PVDF membrane was probed with 1 µg/mL Human GADD45a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5510) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for GADD45a was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GADD45 alpha

GADD45 alpha (Growth arrest and DNA damage-inducible protein 45 alpha) is a 19 kDa member of the GADD45 family of proteins. It is ubiquitously expressed, and induced by DNA damage. Upon induction, GADD45 alpha binds to PCNA, stimulates DNA excision repair, and initiates G2 cell cycle arrest. Human GADD45 alpha is 165 amino acids (aa) in length. It is reported to dimerize with GADD45-beta and -gamma, and to homo-dimerize, -trimerize and -tetramerize via residues 33‑61 and 133‑165. There is one splice variant that shows a Ser substitution for aa 14‑49. GADD45 alpha is 55% aa identical to GADD45-beta and -gamma, and shares 94% aa identity with mouse GADD45 alpha.

Long Name

Growth Arrest and DNA Damage Gene alpha

Entrez Gene IDs

1647 (Human); 13197 (Mouse); 25112 (Rat)

Alternate Names

DDIT-1; DDIT1growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DNA damage-inducible transcript-1; GADD45 alpha; GADD45a; GADD45DNA-damage-inducible transcript 1; growth arrest and DNA-damage-inducible 45 alpha; growth arrest and DNA-damage-inducible, alpha