Human Galectin-3BP/MAC-2BP Antibody Summary
Val19-Asp585
Accession # Q08380
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Galectin‑3BP/MAC‑2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-3BP/MAC-2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin-3BP/MAC-2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Human Galectin‑3BP/MAC‑2BP by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3BP/MAC-2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3BP/MAC-2BP
Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.
- Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
- Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
- Sasaki, T. et al. (1998) EMBO J. 17:1606.
- Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
- Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
- Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
- Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
- Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
Product Datasheets
Citations for Human Galectin-3BP/MAC-2BP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Quantitative glycoproteomics reveals new classes of STT3A- and STT3B-dependent N-glycosylation sites
Authors: Natalia A. Cherepanova, Sergey V. Venev, John D. Leszyk, Scott A. Shaffer, Reid Gilmore
Journal of Cell Biology
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Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis
Authors: Aysha Ferdoushi, Xiang Li, Nathan Griffin, Sam Faulkner, M. Fairuz B. Jamaluddin, Fangfang Gao et al.
Frontiers in Oncology
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Extracellular LGALS3BP: a potential disease marker and actionable target for antibody–drug conjugate therapy in glioblastoma
Authors: Beatrice Dufrusine, Emily Capone, Sara Ponziani, Rossano Lattanzio, Paola Lanuti, Francesco Giansanti et al.
Molecular Oncology
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Extracellular vesicles stimulate smooth muscle cell migration by presenting collagen VI
Authors: Kapustin, A;Tsakali, SS;Whitehead, M;Chennell, G;Wu, MY;Molenaar, C;Kutikhin, A;Bogdanov, L;Sinitsky, M;Rubina, K;Clayton, A;Verweij, FJ;Pegtel, DM;Zingaro, S;Lobov, A;Zainullina, B;Owen, D;Parsons, M;Cheney, RE;Warren, D;Humphries, MJ;Iskratsch, T;Holt, M;Shanahan, CM;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Extracellular Vesicles
Applications: Western Blot -
Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers
Authors: GF Jang, JS Crabb, B Hu, B Willard, H Kalirai, AD Singh, SE Coupland, JW Crabb
Cancers, 2021-07-14;13(14):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Targeting Vesicular LGALS3BP by an Antibody-Drug Conjugate as Novel Therapeutic Strategy for Neuroblastoma
Authors: E Capone, A Lamolinara, F Pastorino, R Gentile, S Ponziani, G Di Vittori, D D'Agostino, S Bibbò, C Rossi, E Piccolo, V Iacobelli, R Lattanzio, V Panella, M Sallese, V De Laurenz, F Giansanti, A Sala, M Iezzi, M Ponzoni, R Ippoliti, S Iacobelli, G Sala
Cancers (Basel), 2020-10-15;12(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Mac-2-binding protein glycan isomer enhances the aggressiveness of hepatocellular carcinoma by activating mTOR signaling
Authors: G Dolgormaa, N Harimoto, N Ishii, T Yamanaka, K Hagiwara, M Tsukagoshi, T Igarashi, A Watanabe, N Kubo, K Araki, T Handa, T Yokobori, T Oyama, H Kuwano, K Shirabe
Br. J. Cancer, 2020-07-06;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Galectin 3-binding protein suppresses amyloid-&beta production by modulating &beta-cleavage of amyloid precursor protein
Authors: T Seki, M Kanagawa, K Kobayashi, H Kowa, N Yahata, K Maruyama, N Iwata, H Inoue, T Toda
J. Biol. Chem., 2020-01-29;295(11):3678-3691.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
Authors: JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh
Mol. Cancer Ther, 2017-03-23;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017-03-20;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
Sci Rep, 2016-09-08;6(0):32736.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
J. Virol., 2012-04-11;86(12):6620-31.
Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
Applications: Western Blot -
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
Cancer Res, 2012-03-02;72(9):2228-38.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
Exp. Cell Res., 2010-12-21;317(7):976-93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
J. Biol. Chem., 2008-05-01;283(27):18573-81.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
F508 CFTR interactome remodelling promotes rescue of cystic fibrosis.
Authors: Sandra Pankow, Casimir Bamberger, Diego Calzolari et al.
Nature
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