Human Gas6 Antibody Summary
Asp118-Ala678
Accession # NP_000811
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas6
Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF‑like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).
- Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Stitt, T.N. et al. (1995) Cell 80:661.
- Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
- Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
- Goruppi, S. et al. (1997) FEBS Lett. 415:59.
- Sather, S. et al. (2007) Blood 109:1026.
- Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
- Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
- Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
- Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
- Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
- Gallicchio, M. et al. (2005) Blood 105:1970.
- Caraux, A. et al. (2006) Nat. Immunol. 7:747.
- Wu, Y. et al. (2005) J. Cell Sci. 118:539.
- Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.
Product Datasheets
Citations for Human Gas6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
Nature Medicine, 2022-08-04;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Zika virus infects pericytes in the choroid plexus and enters the central nervous system through the blood-cerebrospinal fluid barrier
Authors: J Kim, B Alejandro, M Hetman, EM Hattab, J Joiner, H Schroten, H Ishikawa, DH Chung
PLoS Pathog., 2020-05-01;16(5):e1008204.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
Authors: DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma
MBio, 2019-07-16;10(4):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Authors: Zizzo G, Hilliard B, Monestier M, Cohen P
J Immunol, 2012-08-31;189(7):3508-20.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Activation of the AXL kinase causes resistance to EGFR-targeted therapy in lung cancer.
Authors: Zhang Z, Lee JC
Nat. Genet., 2012-07-01;44(8):852-860.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-P, Western Blot -
Differential expression of Axl and Gas6 in renal cell carcinoma reflecting tumor advancement and survival.
Authors: Gustafsson A, Martuszewska D, Johansson M, Ekman C, Hafizi S, Ljungberg B, Dahlback B
Clin. Cancer Res., 2009-06-30;15(14):4742-9.
Species: Human
Sample Types: Serum
Applications: ELISA Development -
Development of a novel immunoassay for the assessment of plasma Gas6 concentrations and their variation with hormonal status.
Authors: Clauser S, Peyrard S, Gaussem P, Crespin M, Emmerich J, Aiach M, Borgel D
Clin. Chem., 2007-08-23;53(10):1808-13.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Gas6 Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Human Gas6 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
